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| Status |
Public on Sep 04, 2020 |
| Title |
miCLIP-seq of postnatal day 0 (P0) normal mouse skin epithelial cells |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We employed miCLIP-seq to profile the location and extent of m6A in the Poly(A)+ transcriptome. Skin epithelial cells were isolated from wild-type P0 neonates by FACS. Total RNA was extracted by TRIzol-LS and Poly(A)+ RNA was extracted with Dynabeads™ mRNA Purification Kit (Thermo-Fisher, 61006). Input and miCLIP libraries was prepared from 3 biological replicates with each containing RNA isolated from 3 litters of neonates. Libraries were sequenced on the Illumina Hi-seq platform to generate paired-ended 50 bp reads. Sequencing data was processed as described in (Geula et al., 2015).
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| Overall design |
Examination of transcriptome-wide m6A profile in normal skin epithelial cells.
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| |
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| Contributor(s) |
Xi L, Fuchs E |
| Citation(s) |
32845239 |
| |
| Submission date |
Mar 24, 2020 |
| Last update date |
Sep 04, 2020 |
| Contact name |
Tom Samuel Carroll |
| E-mail(s) |
thomas.carroll@rockefeller.edu
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| Organization name |
The Rockefeller University
|
| Department |
Bioinformatics
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| Lab |
Bioinformatics
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA614972 |
| SRA |
SRP253908 |
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