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Series GSE147496 Query DataSets for GSE147496
Status Public on Dec 08, 2020
Title RNA-seq of primary mouse B cell progenitor populations after activation with LPS
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Naive resting B-cells were purified from bone marrow from C57BL/6 mice using flow cytometry sorting. Naive B cells were activated with LPS and harvested 3 hours, 10 hours or 33 hours after the LPS treatment. Expanded activated B cells (CD22+ CD138-) and plasmablasts (CD22- CD138+) were also harvested on day 4 post-stimulation.
 
Overall design Two biological replicates were prepared of naive B cells, activated B cells after each LPS treatment time and plasmablasts. All 12 samples were RNA-seq profiled by sequencing on an Illumina NextSeq 500 to produce 81bp paired-end reads.
 
Contributor(s) Coughlan HD, Smyth GK
Citation(s) 33637722
Submission date Mar 24, 2020
Last update date Mar 09, 2021
Contact name Gordon K Smyth
E-mail(s) smyth@wehi.edu.au
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
URL http://www.wehi.edu.au
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM4432236 Naive-I
GSM4432237 Naive-II
GSM4432238 Ab-I
This SubSeries is part of SuperSeries:
GSE147497 RNA-seq and in situ Hi-C of primary mouse B cell progenitor populations after activation with LPS
Relations
BioProject PRJNA614985
SRA SRP253917

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE147496_GenewiseCounts.tsv.gz 491.4 Kb (ftp)(http) TSV
GSE147496_RAW.tar 2.1 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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