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Series GSE147723 Query DataSets for GSE147723
Status Public on Dec 06, 2023
Title ChIP-seq analysis comparing WT, TAp63-/-, and ΔNp63-/- primary murine keratinocytes
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The p53 family member TP63 encodes two sets of isoforms, TAp63 and ∆Np63 isoforms, which are characterized by different N-termini and have diverse biological functions in epidermal morphogenesis and in cancer. In the skin, where their activities are best characterized, TAp63 prevents premature aging by regulating cellular senescence and genomic stability of stem cells, while ∆Np63 controls terminal differentiation of the basal cells in the epidermis. This functional diversity is surprising given that these isoforms share a high degree of similarity, including an identical DNA binding domain. To understand the mechanisms involved in the transcriptional programs leading to these diverse biological functions, we performed genome-wide analyses using p63-ChIP-seq and RNA-seq of TAp63-/- and ∆Np63-/- compared to wild-type primary mouse epidermal cells. Our data indicate that TAp63 and ∆Np63 recognize significantly different response elements on DNA and can physically and functionally interact with distinct transcription factors for the downstream regulation of their target genes. Our findings unveil previously uncharacterized transcriptomes activated by the p63 isoforms to regulate diverse biological functions in epidermal morphogenesis and homeostasis and cancer.
Overall design We performed a ChIP-seq analysis comparing differential bindings of p63 in 3 samples: WT, TAp63-/-, and ΔNp63-/- primary murine keratinocytes.
Contributor(s) Napoli M, Flores ER, Coarfa C
Citation(s) 38165157
Submission date Mar 30, 2020
Last update date Feb 14, 2024
Contact name Elsa Renee Flores
Organization name Moffit Cancer Center
Department Department of Molecular Oncology
Lab The Flores Lab
Street address 12902 USF Magnolia Drive
City Tampa
State/province FL
ZIP/Postal code 33612
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM4443816 Wild-type ChIP-Seq mouse keratinocytes
GSM4443817 TAp63-/- ChIP-Seq mouse keratinocytes
GSM4443818 ΔNp63-/- ChIP-Seq mouse keratinocytes
BioProject PRJNA616251
SRA SRP254540

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Supplementary file Size Download File type/resource
GSE147723_RAW.tar 250.0 Mb (http)(custom) TAR (of TDF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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