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Series GSE148328 Query DataSets for GSE148328
Status Public on Dec 07, 2020
Title The transcriptional coactivator CBP/p300 is an evolutionarily conserved node that promotes longevity in response to mitochondrial stress [ChIP-seq]
Organism Caenorhabditis elegans
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Organisms respond to mitochondrial stress by activating multiple defense pathways including the mitochondrial unfolded protein response (UPRmt). However, how different layers of UPRmt regulators are orchestrated to transcriptionally activate the stress responses remains largely unknown. Here we identified CBP-1, the worm ortholog of the mammalian acetyltransferases CBP/p300, as an essential regulator for UPRmt activation, as well as for mitochondrial stress-induced immune response, amyloid-β aggregation reduction and lifespan extension in Caenorhabditis elegans. Mechanistically, CBP-1 acts downstream of histone demethylases, JMJD-1.2/JMJD-3.1, and upstream of UPRmt transcription factors including ATFS-1, to systematically induce a broad spectrum of UPRmt genes and execute multiple beneficial functions. In mouse and human populations, transcript levels of CBP/p300 positively correlate with UPRmt transcripts and longevity. Furthermore, CBP/p300 inhibition disrupts, while forced expression of p300 is sufficient to activate, the UPRmt in mammalian cells. These results highlight an evolutionarily conserved mechanism that determines mitochondrial stress response, and promotes health and longevity through CBP/p300.
Overall design N2 worms carrying a hsp-6::GFP reporter fed with control or cco-1 RNAi, and ChIP with H3K18Ac or H3K27Ac
Contributor(s) Li TY, Bou Sleiman MS, Li H, Gao AW, Mottis A, Bachmann A, Alam GE, Schoonjans K, Auwerx J
Citation(s) 33718883
Submission date Apr 08, 2020
Last update date Mar 23, 2021
Contact name Terytty Yang Li
Organization name EPFL
Street address EPFL SV IBI-SV LISP AI 1145
City Lausanne
ZIP/Postal code 1015
Country Switzerland
Platforms (1)
GPL25145 BGISEQ-500 (Caenorhabditis elegans)
Samples (6)
GSM4460499 N2 + ev, 1
GSM4460500 N2 + ev, 2
GSM4460501 N2 + 50% ev + 50% cco-1 RNAi,1
This SubSeries is part of SuperSeries:
GSE131616 The transcriptional coactivator CBP/p300 is an evolutionarily conserved node that promotes longevity in response to mitochondrial stress
BioProject PRJNA623903
SRA SRP255746

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Supplementary file Size Download File type/resource
GSE148328_Processed_ChIP-seq_data_Worm_TYL.xlsx 3.6 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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