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Series GSE148360 Query DataSets for GSE148360
Status Public on Jul 30, 2020
Title Non-neuronal expression of SARS-CoV-2 entry genes in the olfactory system suggests mechanisms underlying COVID-19-associated anosmia (scRNA-seq: olfactory bulb)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A subset of COVID-19 patients exhibit altered olfactory function. Here we analyze bulk and single cell RNA-Seq datasets to identify cell types in the olfactory epithelium and olfactory bulb that express cell entry molecules that mediate infection by SARS-CoV-2 (CoV-2), the causal agent in COVID-19. We find that samples from whole olfactory mucosa in species including mouse and human express two key genes involved in CoV-2 entry, ACE2 and TMPRSS2. However, neither olfactory sensory neurons nor olfactory bulb neurons express these genes, which are instead expressed in support cells, stem cells, and perivascular cells. These findings suggest that CoV-2 infection of non-neuronal cell types leads to anosmia and related disturbances in odor perception in COVID-19 patients.
 
Overall design Two 15 min Drop-seq runs from a single dissociation of the olfactory bulbs were obtained from 2 mice. Two such dissociations were performed, giving 4 total replicates. Additional Drop-Seq data from the olfactory mucosa can be found in GSE151346.
 
Contributor(s) Brann DH, Tsukahara T, Datta SR
Citation(s) 32937591
Submission date Apr 09, 2020
Last update date Sep 19, 2020
Contact name David Brann
Organization name Harvard Medical School
Department Neurobiology
Lab Datta Lab
Street address 220 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM4462798 OB_121316_run1
GSM4462799 OB_121316_run2
GSM4462800 OB_121516_run1
Relations
BioProject PRJNA624068
SRA SRP255864

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE148360_Datta_integratedOB_UMAP_noDoublets.csv.gz 535.3 Kb (ftp)(http) CSV
GSE148360_Linnarsson_integratedOB_UMAP_noDoublets.csv.gz 400.4 Kb (ftp)(http) CSV
GSE148360_OB_2016Dec_all_counts.csv.gz 42.2 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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