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Series GSE148460 Query DataSets for GSE148460
Status Public on Jun 01, 2020
Title Role of human CYP2B6 in vivo in diet-induced obesity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the role of human CYP2B6 in vivo under high-fat diet conditions, we compared our previously developed Cyp2b triple knockout mouse lacking Cyp2b9, Cyp2b10, and Cyp2b13 (via CRISPER/Cas9; Cyp2b-null), to our newly developed humanized-CYP2B6-transgenic (hCYP2B6-Tg) mouse model (on a Cyp2b-null background). hCYP2B6-Tg and Cyp2b-null mice were fed a high-fat diet consisting of 60% fat for 16 weeks. RNA was extracted from the livers of female and male mice from both treatment groups and used for RNA seqencing. RNAseq and in gene ontology (GO) analysis indicate human CYP2B6 is important in protein processing and phosphorylation, hepatic circadian regulation, and lipid metabolism under HFD conditions.Circadian rhythm-associated genes were up-regulated in HFD-fed hCYP2B6-Tg mice compared to Cyp2b-null mice. Circadian regulation plays an important role in liver metabolism and metabolic disease. In addition, female hCYP2B6-Tg mice had several down-regulated genes involved in lipid metabolism compared to Cyp2b-null mice, notably Angptl8 (logFC = -1.24), a critical modulator of lipid metabolism that has been associated with metabolic disease. Genes identified by RNAseq analysis may contribute to the overall healthier metabolic profile of HFD-fed humanized mice compared to Cyp2b-null mice.
 
Overall design Use RNA-sequencing to investigate the role of human CYP2B6 in diet-induced obesity on a transcriptomic level, by comparing the livers of Cyp2b-null and hCYP2B6-Tg mice fed a high-fat diet for 16 weeks using Illumina technology.
 
Contributor(s) Baldwin WS, Heintz MM, Noorai RE
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Submission date Apr 10, 2020
Last update date Jun 01, 2020
Contact name William S Baldwin
E-mail(s) baldwin@clemson.edu
Phone 915-491-7366
Organization name Clemson University
Department Biological Sciences
Lab Dr. William Baldwin
Street address 230 Parkway Dr
City Clemson
State/province South Carolina
ZIP/Postal code 29634
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (16)
GSM4471971 NULL_1F
GSM4471972 NULL_3F
GSM4471973 NULL_4F
Relations
BioProject PRJNA624315
SRA SRP255965

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE148460_Rawcounts_CYP2B6_HFD_study.txt.gz 632.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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