 |
 |
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Aug 02, 2020 |
Title |
Single-cell profiling reveals Colonic CD8 Topography in IBD -hIL26 Mouse RNA-Seq |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Aim: To assess the effect of IL-26 on mouse colonic tissue at steady state and in DSS-induced colitis through next-generation RNA sequencing of bulk RNA from colonic tissue. Results: We demonstrate an anti-inflammatory effect of IL-26 at both steady-state and after induction of DSS-colitis, through suppression of both cell recruitment and activation pathways.
|
|
|
Overall design |
Methods: Mice do not express IL-26, but do express the putative IL-26 receptor (IL10RB/IL20RA). The study therefore utilized WT (C57BL/6 B6) or TG mice (C57BL/6 expressing human IL26 BAC transgene) littermate mice housed in pathogen-free conditions with regularized day-night cycles and ad-libitum access to sterile food and water. These mice were either assessed at age 20-24 weeks (steady-state,for any spontaneous inflammation) or exposed to 2.5% DSS at age 10-14 weeks for 6 days, with and without exposure to anti-IL26 antibody or isotype control antibody. Bulk RNA was extracted from the colonic tissue of these mice, enriched for mRNA with strand-specific information, and analyzed with next-generation RNA sequencing.
|
|
|
Contributor(s) |
Corridoni D, Antanaviciute A, Ishikawa D, Hatano R, Morimoti C, Jagielowicz M |
Citation(s) |
32747828 |
|
Submission date |
Apr 11, 2020 |
Last update date |
Nov 16, 2020 |
Contact name |
Alison Simmons |
E-mail(s) |
alison.simmons@ndm.ox.ac.uk
|
Organization name |
University of Oxford
|
Department |
The MRC Weatherall Institute of Molecular Medicine
|
Street address |
John Radcliffe Hospital/Headley Way
|
City |
Oxford |
ZIP/Postal code |
OX3 9DS |
Country |
United Kingdom |
|
|
Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
Samples (15)
|
GSM4472569 |
RNA-Seq Sample3, Colon, WT, Control |
GSM4472570 |
RNA-Seq Sample4, Colon, WT, Control |
GSM4472571 |
RNA-Seq Sample7, Colon, WT, DSS |
GSM4472572 |
RNA-Seq Sample6, Colon, WT, DSS |
GSM4472573 |
RNA-Seq Sample10, Colon, WT, DSS |
GSM4472574 |
RNA-Seq Sample14, Colon, TG, Control |
GSM4472575 |
RNA-Seq Sample19, Colon, TG, DSS |
GSM4472576 |
RNA-Seq Sample22, Colon, TG, DSS |
GSM4472577 |
RNA-Seq Sample13, Colon, TG, Control |
GSM4472578 |
RNA-Seq Sample20, Colon, TG, DSS |
GSM4472579 |
RNA-Seq Sample21, Colon, TG, DSS |
GSM4472580 |
RNA-Seq Sample1, Colon, WT, Control |
|
Relations |
BioProject |
PRJNA624778 |
SRA |
SRP256227 |
Supplementary file |
Size |
Download |
File type/resource |
GSE148505_raw_read_count_matrix.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
|
|
|
|
 |