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| Status |
Public on Oct 06, 2020 |
| Title |
Systemic induction of phospholipid-based signaling in leaves of arbuscular mycorrhizal rice plants |
| Organisms |
Oryza sativa; Funneliformis mosseae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Most land plants form beneficial associations with arbuscular mycorrhizal (AM) fungi which improves mineral nutrition, mainly phosphorus and nitrogen in the host plant in exchange for photosynthetically fixed carbon. Most of our knowledge on the AM symbiosis derives from dicotyledonous species. We show that inoculation with the AM fungus Funneliformis mossease stimulates growth and increases Pi content in leaves of the rice cultivar Loto (O. sativa ssp japonica). Although rice is a host for AM fungi, the molecular mechanism underlying the AM symbiosis, in particular the systemic transcriptional responses of shoots to AM inoculation, remain largely elusive. Transcript profiling of the shoots indicated the systemic induction of genes involved in the biosynthesis of phospholipids (phosphoinositides, inositol polyphosphates) and down-regulation of non-phosphorus lipids (galactolipids, sulfolipids) in leaves of mycorrhizal rice. Regulation of phospholipid biosynthesis genes appears to be coordinated with a reduced expression of genes involved in jasmonic acid and ethylene biosynthesis and signaling. Genes involved in phosphate starvation responses and remobilization of Pi were also found to be down-regulated in leaves of mycorrhizal rice. These results demonstrated that the AM symbiosis is accompanied by complex alterations in gene expression in shoots which are potentially important to maintain a stable symbiotic relationship in rice plants.
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| Overall design |
6 samples in total: wild-type rice plants (Oryza sativa, subsp japonica cv. Loto) with 2 treatments (inoculated or not with the arbuscular micorrhyzae fungus Funneliformis mosseae), 3 biological replicates each treatment
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| Contributor(s) |
Campo S, San Segundo B |
| Citation(s) |
32985595 |
| |
| Submission date |
Apr 13, 2020 |
| Last update date |
Oct 06, 2020 |
| Contact name |
Blanca San Segundo |
| E-mail(s) |
blanca.sansegundo@cragenomica.es
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| Organization name |
Center for Research in Agricultural Genomics (CRAG)
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| Department |
Plant Responses to Stress
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| Street address |
Edifici CRAG - Campus UAB
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| City |
Bellaterra |
| State/province |
Barcelona |
| ZIP/Postal code |
08193 |
| Country |
Spain |
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| Platforms (2) |
| GPL19290 |
Illumina HiSeq 2500 (Oryza sativa) |
| GPL28382 |
Illumina HiSeq 2500 (Funneliformis mosseae; Oryza sativa) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA625053 |
| SRA |
SRP256285 |
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