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| Status |
Public on Dec 31, 2022 |
| Title |
microRNA Expression Study of chicken CD3-28-4+ intraepithelial lymphocyte natural killer cells infected with very virulent Infectious Bursal Disease Virus |
| Organism |
Gallus gallus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Infectious bursal disease is an acute immunosuppressive viral disease which significantly affect the economic in poultry industrial. Infectious bursal disease virus (IBDV) infection was known to destroy B lymphocytes, activate macrophage and T lymphocytes, but there are limited studies on the impact of IBDV infection on chicken intraepithelial Natural killer (IEL-NK) cells.
The main objective of this study is to determine the innate immune response of chicken IEL-NK cells towards very virulent IBDV (vvIBDV) infection. Specific pathogen free (SPF) chickens were infected with vvIBDV for 0, 1 and 3 dpi. The IEL-NK cells were isolated and enriched from total IEL cells using the 28.4+ antibodies. The microRNA sequencing was conducted in MiSeq system and approximately 1.6 to 3.6 million single reads generated per sample. The differential expression analysis was performed on the miRNA-Seq data. there are 35 and 16 sRNAs were differentially expressed at 1 and 3 dpi, respectively. The SNORD101, gga-miR-222a and gga-miR-221-3p were up-regulated on Day 1 and 3 whereas gga-miR-30a-50, gga-miR-142-5p, gga-miR-32-5p and gga-miR-146b-5p were down-regulated on both days.
gga-miR-217-5p and gga-miR-222b-5p with the highest fold change were involved in the regulation of tumor growth and apoptosis by targeting the MAPK signaling pathway. The vvIBDV replication was suppressed by overexpression of gga-miR-21 at 3 dpi. Some of the miRNAs were up-regulated with unknown reasons but the same findings were showed in other publications such as gga-miR-3538 and gga-miR-2954 which were up-regulated after being infected by other viruses, but the roles of these miRNA are not clear.
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| Overall design |
The miRNA-Seq data for 3 groups of samples (uninfected chicken, chicken infected by vvIBDV for 1 day and chicken infected by vvIBDV for 3 days) in triplicate was generated using MiSeq
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| Contributor(s) |
Yee BS, Keong YS |
| Citation missing |
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| |
| Submission date |
Apr 16, 2020 |
| Last update date |
Jan 02, 2023 |
| Contact name |
Boo Sook Yee |
| E-mail(s) |
rinoa1685@gmail.com
|
| Phone |
+60128319568
|
| Organization name |
Universiti Putra Malaysia
|
| Department |
Institute of Bioscience
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| Lab |
Laboratory of Vaccine & Immunotherapeutics
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| Street address |
Universiti Putra Malaysia
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| City |
Serdang |
| State/province |
Selangor |
| ZIP/Postal code |
43400 |
| Country |
Malaysia |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA625688 |
| SRA |
SRP256563 |
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