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| Status |
Public on Dec 07, 2020 |
| Title |
Post-transcriptional tuning of FGF signaling mediates neural crest induction |
| Organism |
Gallus gallus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
Ectodermal patterning is required for the establishment of multiple components of the vertebrate body plan. Previous studies have demonstrated that precise combinations of extracellular signals determine the induction of neural or neural crest progenitors. Yet, we still have a limited understanding of how the response to inductive signals is optimized to generate the proper transcriptional output in target cells. Here we show that post-transcriptional attenuation of signaling gradients is essential for the formation of the neural crest. We found that neural crest cells display enhanced expression of Dicer, which promotes the maturation of a set of cell-type-specific miRNAs. These miRNAs target multiple components of the FGF signaling pathway, a central player in the process of neural induction in avian embryos. Loss of neural crest miRNAs prevented the attenuation of this neuralizing signal, leading to the expansion of the neural plate at the expense of neural crest cells. Thus, the post-transcriptional attenuation of FGF signaling is pre-requisite for neural crest specification. These findings demonstrate how post-transcriptional repression may reshape signaling gradients to set the boundaries between distinct spatial domains of progenitor cells.
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| Overall design |
Small RNA-sequencing data for FACS sorted chicken neural plate and neural crest cells at one developmental timepoint (HH8).
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| Contributor(s) |
Simoes-Costa M, Copeland J |
| Citation missing |
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| |
| Submission date |
May 06, 2020 |
| Last update date |
Dec 07, 2020 |
| Contact name |
Marcos Simoes-Costa |
| E-mail(s) |
simoescosta@cornell.edu
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| Organization name |
Cornell University
|
| Department |
Molecular Biology and Genetics
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| Lab |
Simoes-Costa Lab
|
| Street address |
526 Campus Rd
|
| City |
Ithaca |
| State/province |
New York |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19787 |
Illumina NextSeq 500 (Gallus gallus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA630815 |
| SRA |
SRP260343 |
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