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Series GSE150007 Query DataSets for GSE150007
Status Public on Dec 07, 2020
Title Post-transcriptional tuning of FGF signaling mediates neural crest induction
Organism Gallus gallus
Experiment type Non-coding RNA profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Ectodermal patterning is required for the establishment of multiple components of the vertebrate body plan. Previous studies have demonstrated that precise combinations of extracellular signals determine the induction of neural or neural crest progenitors. Yet, we still have a limited understanding of how the response to inductive signals is optimized to generate the proper transcriptional output in target cells. Here we show that post-transcriptional attenuation of signaling gradients is essential for the formation of the neural crest. We found that neural crest cells display enhanced expression of Dicer, which promotes the maturation of a set of cell-type-specific miRNAs. These miRNAs target multiple components of the FGF signaling pathway, a central player in the process of neural induction in avian embryos. Loss of neural crest miRNAs prevented the attenuation of this neuralizing signal, leading to the expansion of the neural plate at the expense of neural crest cells. Thus, the post-transcriptional attenuation of FGF signaling is pre-requisite for neural crest specification. These findings demonstrate how post-transcriptional repression may reshape signaling gradients to set the boundaries between distinct spatial domains of progenitor cells.
Overall design Small RNA-sequencing data for FACS sorted chicken neural plate and neural crest cells at one developmental timepoint (HH8).
Contributor(s) Simoes-Costa M, Copeland J
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Submission date May 06, 2020
Last update date Dec 07, 2020
Contact name Marcos Simoes-Costa
Organization name Cornell University
Department Molecular Biology and Genetics
Lab Simoes-Costa Lab
Street address 526 Campus Rd
City Ithaca
State/province New York
ZIP/Postal code 14853
Country USA
Platforms (1)
GPL19787 Illumina NextSeq 500 (Gallus gallus)
Samples (10)
GSM4520632 HH8 NC rep1
GSM4520633 HH8 NC rep2
GSM4520634 HH8 NC rep3
BioProject PRJNA630815
SRA SRP260343

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Supplementary file Size Download File type/resource
GSE150007_DESeq2_results.csv.gz 24.1 Kb (ftp)(http) CSV
GSE150007_RNA-seq_DESeq2_results_NCvNP.csv.gz 617.8 Kb (ftp)(http) CSV
GSE150007_RNA-seq_raw_counts_all_samples.csv.gz 107.9 Kb (ftp)(http) CSV
GSE150007_Raw_counts.csv.gz 7.8 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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