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Series GSE150391 Query DataSets for GSE150391
Status Public on Jul 09, 2021
Title POU4F3 Pioneer Activity Enables ATOH1 to Drive Diverse Mechanoreceptor Differentiation Through a Feed-Forward Epigenetic Mechanism
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary During embryonic development, hierarchical cascades of transcription factors interact with lineage specific chromatin structures to control the sequential steps in the differentiation of specialized cell types. While examples of transcription factor cascades have been well documented, the mechanisms underlying developmental changes in accessibility of cell type-specific enhancers remain poorly understood. Here we show that the transcriptional ‘master regulator’, ATOH1 – which is necessary for the differentiation of two distinct mechanoreceptor cell types, hair cells in the inner ear, and Merkel cells of the epidermis – is unable to access much of its target enhancer network in the progenitor populations of either cell type when it first appears, imposing a block to further differentiation. This block is overcome by a feed-forward mechanism in which ATOH1 first stimulates expression of POU4F3, which subsequently acts as a pioneer factor to provide access to closed ATOH1 enhancers, allowing hair cell and Merkel cell differentiation to proceed. Our analysis also indicates the presence of both shared and divergent ATOH1/POU4F3-dependent, enhancer networks in hair cells and Merkel cells. These cells share a deep developmental lineage relationship, deriving from their common epidermal origin, and suggesting that this feed-forward mechanism preceded the evolutionary divergence of these very different mechanoreceptive cell types.

Overall design Refer to individual Series
Citation(s) 34266958
Submission date May 12, 2020
Last update date Jul 16, 2021
Contact name Neil Segil
Phone 3234421549
Organization name University of Southern California
Department BCC
Lab 503K
Street address 1425 san pablo st, BCC 503K
City Los Angeles
State/province CA
ZIP/Postal code 90033
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (68)
GSM4544622 P1CGP_ATAC_100_cell_rep1
GSM4544623 P1CGP_ATAC_100_cell_rep2
GSM4544624 P1CGP_ATAC_500_cell_rep1
This SuperSeries is composed of the following SubSeries:
GSE150275 µATACseq: ATACseq with as few as 100 cells
GSE150279 Using Mouse Embryonic Fibroblast to test the pioneer factor activity of POU4F3
GSE150386 ATACseq, CutnRun and RNAseq data from sensory progenitors and hair cells, from wild type and Pou4f3-mutant cochlea
BioProject PRJNA631963

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150391_RAW.tar 162.8 Mb (http)(custom) TAR (of NARROWPEAK, TXT)
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