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Series GSE150559 Query DataSets for GSE150559
Status Public on May 27, 2020
Title Direct and indirect targets of Arabidopsis ABI3: genome wide binding sites [ChIP-chip]
Organism Arabidopsis thaliana
Experiment type Genome variation profiling by genome tiling array
Summary ABSCISIC ACID INSENSITIVE3 (ABI3) is a transcription factor that is a member of the B3 domain family and is involved in seed development. To understand how it controls development, we mapped genome-wide binding sites by ChIP-chip and combined this data with transcriptomic data obtained by microarrays.
Ran Tian, Fangfang Wang, Qiaolin Zheng, Venus Niza, A. Bruce Downie and Sharyn E. Perry (2020)
Overall design ABI3 was expressed by its native promoter and included a C-terminal 10x c-myc tag. This transgene was able to rescue the abi3-5 mutant. A second transgene (35S:AGL15) allowed establishment of stable somatic embryo cultures. ChIP was performed using anti-c-myc antibody (Myc-Tag (9B11) Mouse mAb; Cell Signaling 2276S) on tissue expressing ABI3-myc (three biological replicates) and on three controls of tissue without c-myc antibody.
Contributor(s) Perry S
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Submission date May 14, 2020
Last update date May 29, 2020
Contact name Sharyn E Perry
Phone 859-218-0732
Organization name University of Kentucky
Department Plant and Soil Sciences
Street address 1405 Veterans Dr.
City Lexington
State/province KY
ZIP/Postal code 40546-0312
Country USA
Platforms (1)
GPL10977 [At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array
Samples (6)
GSM4552321 ABI3-myc IP with anti-myc antibody, biological rep 1
GSM4552322 ABI3-myc IP with anti-myc antibody, biological rep 2
GSM4552323 ABI3-myc IP with anti-myc antibody, biological rep 3
This SubSeries is part of SuperSeries:
GSE150561 Direct and indirect targets of Arabidopsis ABI3: genome wide binding sites
BioProject PRJNA632775

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150559_RAW.tar 379.2 Mb (http)(custom) TAR (of BAR, CEL, TXT)
Processed data provided as supplementary file

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