|
| Status |
Public on May 27, 2020 |
| Title |
Direct and indirect targets of Arabidopsis ABI3: genome wide binding sites [ChIP-chip] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome variation profiling by genome tiling array
|
| Summary |
ABSCISIC ACID INSENSITIVE3 (ABI3) is a transcription factor that is a member of the B3 domain family and is involved in seed development. To understand how it controls development, we mapped genome-wide binding sites by ChIP-chip and combined this data with transcriptomic data obtained by microarrays.
Ran Tian, Fangfang Wang, Qiaolin Zheng, Venus Niza, A. Bruce Downie and Sharyn E. Perry (2020)
|
| |
|
| Overall design |
ABI3 was expressed by its native promoter and included a C-terminal 10x c-myc tag. This transgene was able to rescue the abi3-5 mutant. A second transgene (35S:AGL15) allowed establishment of stable somatic embryo cultures. ChIP was performed using anti-c-myc antibody (Myc-Tag (9B11) Mouse mAb; Cell Signaling 2276S) on tissue expressing ABI3-myc (three biological replicates) and on three controls of tissue without c-myc antibody.
|
| |
|
| Contributor(s) |
Perry S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 14, 2020 |
| Last update date |
May 29, 2020 |
| Contact name |
Sharyn E Perry |
| E-mail(s) |
sperr2@uky.edu
|
| Phone |
859-218-0732
|
| Organization name |
University of Kentucky
|
| Department |
Plant and Soil Sciences
|
| Street address |
1405 Veterans Dr.
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40546-0312 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10977 |
[At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array |
|
| Samples (6)
|
| GSM4552321 |
ABI3-myc IP with anti-myc antibody, biological rep 1 |
| GSM4552322 |
ABI3-myc IP with anti-myc antibody, biological rep 2 |
| GSM4552323 |
ABI3-myc IP with anti-myc antibody, biological rep 3 |
| GSM4552324 |
Control immunoprecipitation, biological rep 1 |
| GSM4552325 |
Control immunoprecipitation, biological rep 2 |
| GSM4552326 |
Control immunoprecipitation, biological rep 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE150561 |
Direct and indirect targets of Arabidopsis ABI3: genome wide binding sites |
|
| Relations |
| BioProject |
PRJNA632775 |
Less...
More...