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Series GSE150584 Query DataSets for GSE150584
Status Public on Sep 08, 2022
Title Gene expression profile of primary CLL cells transduced with a retrovirus expressing mutated NOTCH1
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary In CLL, NOTCH1 is the most commonly mutated gene at diagnosis and it is associated with a poor outcome. The mechanisms underlying how NOTCH1 contributes to disease progression, resistance to treatment and Richter’s transformation remain largely unknown. The main goal of this study is to compare the gene expression profile of primary CLL cells transduced with the intracellular part of NOTCH1 lacking of the PEST domain vs cells transduced with an empty vector. Additionally, patients with the Trisomy 12 abnormality are included in this study to clarify the role of the mutations in this specific sub-group of CLL patients that are strongly associated with NOTCH1 mutations.
Overall design primary CLL cells from 13 patients were isolated through cell sorting 4 days post transdusction with a NOTCH1ΔPEST or an empty retroviral vector
Contributor(s) Mangolini M, Adam G, Mohorianu I, Ringshausen I
Citation(s) 36266281
Submission date May 14, 2020
Last update date Oct 31, 2022
Contact name Irina Mohorianu
Organization name University of Cambridge
Department Wellcome-MRC Cambridge Stem Cell Institute
Street address Puddicombe Way
City Cambridge
ZIP/Postal code CB2 0AW
Country United Kingdom
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (52)
GSM4552705 P1_CNT.replicate1
GSM4552706 P1_CNT.replicate2
GSM4552707 P1_Notch.replicate1
This SubSeries is part of SuperSeries:
GSE150610 Gene expression profile of primary CLL cells transduced with a mutated NOTCH1
BioProject PRJNA632867
SRA SRP261626

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Supplementary file Size Download File type/resource
GSE150584_normalised_expression.txt.gz 3.2 Mb (ftp)(http) TXT
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