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Series GSE150994 Query DataSets for GSE150994
Status Public on Mar 18, 2021
Title Next Generation Sequencing Facilitates Quantitative Analysis of HaCaT and OSM-treated HaCaT Transcriptomes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling (RNA-seq) between HaCaT cell and OSM-treated HaCaT cells
Methods: mRNA profiles of HaCaT cells and OSM-treated HaCaT cells were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.
 
Overall design mRNA profiles of HaCaT cells and OSM-treated HaCaT cells
 
Contributor(s) Liu J, Rong Z
Citation(s) 33502684
Submission date May 21, 2020
Last update date Mar 18, 2021
Contact name Jun Liu
E-mail(s) alexliu@smu.edu.cn
Organization name Southern Medical University
Department Dermatology Hospital
Street address No.2 Lujing Road
City Guangzhou
State/province Guangdong
ZIP/Postal code 510091
Country China
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM4563814 HaCaT1
GSM4563815 HaCaT2
GSM4563816 HaCaT3
Relations
BioProject PRJNA634341
SRA SRP262634

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150994_RNAseqAnalysis.xlsx 2.6 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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