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Series GSE151158 Query DataSets for GSE151158
Status Public on Jul 08, 2020
Title Transcriptional analysis of non-fibrotic NAFLD progression
Platform organism Homo sapiens
Sample organisms Homo sapiens; blank sample
Experiment type Expression profiling by array
Summary Background & Aims: Non-alcoholic steatohepatitis (NASH), a subtype of non-alcoholic fatty liver disease (NAFLD) that can lead to fibrosis, cirrhosis, and hepatocellular carcinoma, is characterized by hepatic inflammation. Despite evolving therapies aimed to ameliorate inflammation in NASH, the transcriptional changes that lead to inflammation progression in NAFLD remain poorly understood. The aim of this study is to define transcriptional changes in early, non-fibrotic NAFLD using a biopsy-proven non-fibrotic NAFLD cohort. Methods: We extracted RNA from liver tissue of 40 patients with biopsy-proven NAFLD based on NAFLD Activity Score (NAS) (23 with NAS ≤3, 17 with NAS ≥5) and 21 healthy controls and compared changes in expression of 594 genes involved in innate immune function. Results: Compared to healthy controls, NAFLD patients with NAS ≥5 had differential expression of 211 genes, while those with NAS ≤3 had differential expression of only 14 genes. Notably, osteopontin (SPP1) (3.74-fold in NAS ≤3, 8.28-fold in NAS ≥5) and CXCL10 (2.27-fold in NAS ≤3, 8.28-fold in NAS ≥5) gene expression were significantly upregulated with histologic progression of NAFLD.
 
Overall design We extracted RNA from liver tissue of 40 patients with biopsy-proven NAFLD based on NAFLD Activity Score (NAS) (23 with NAS ≤3, 17 with NAS ≥5) and 21 healthy controls (protocol biopsy obtained during living liver donation) and compared changes in expression of 594 genes involved in innate immune function
 
Contributor(s) Kriss M, Golden-Mason L, Kaplan J, Richt C, Rosen HR
Citation(s) 32730345
Submission date May 25, 2020
Last update date Aug 14, 2020
Contact name Michael S Kriss
E-mail(s) Michael.Kriss@cuanschutz.edu
Organization name University of Colorado School of Medicine
Street address 12700 E 19th Ave, MS-B146
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
 
Platforms (1)
GPL28577 NanoString Human Immunology v2 Code Set (NS_Immunology_v2_C2328+PLS_Golden_1_C5164)
Samples (66)
GSM4567420 Liver_RNA-01_NAS5
GSM4567421 Liver_RNA-02_NAS5
GSM4567422 Liver_RNA-03_NAS5
Relations
BioProject PRJNA634922

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151158_RAW.tar 500.0 Kb (http)(custom) TAR (of RCC)
Processed data included within Sample table

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