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Series GSE151346 Query DataSets for GSE151346
Status Public on Jul 30, 2020
Title Non-neuronal expression of SARS-CoV-2 entry genes in the olfactory system suggests mechanisms underlying COVID-19-associated anosmia (scRNA-seq: olfactory mucosa)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A subset of COVID-19 patients exhibit altered olfactory function. Here we analyze bulk and single cell RNA-Seq datasets to identify cell types in the olfactory epithelium and olfactory bulb that express cell entry molecules that mediate infection by SARS-CoV-2 (CoV-2), the causal agent in COVID-19. We find that samples from whole olfactory mucosa in species including mouse and human express two key genes involved in CoV-2 entry, ACE2 and TMPRSS2. However, neither olfactory sensory neurons nor olfactory bulb neurons express these genes, which are instead expressed in support cells, stem cells, and perivascular cells. These findings suggest that CoV-2 infection of non-neuronal cell types leads to anosmia and related disturbances in odor perception in COVID-19 patients.
Overall design Eight 15 min Drop-seq runs from dissociated whole olfactory mucosa from five adult male C57BL/6 mice (8–12 weeks-old). Additional Drop-Seq data from the olfactory bulb can be found in GSE148360.
Contributor(s) Brann DH, Tsukahara T, Datta SR
Citation(s) 32937591
Submission date May 28, 2020
Last update date Sep 19, 2020
Contact name David Brann
Organization name Harvard Medical School
Department Neurobiology
Lab Datta Lab
Street address 220 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM4575746 MOE_0105
GSM4575747 MOE_0106
GSM4575748 MOE_0126_1
BioProject PRJNA635577
SRA SRP265068

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Supplementary file Size Download File type/resource
GSE151346_MOE_all_counts.csv.gz 58.2 Mb (ftp)(http) CSV
GSE151346_MOE_metadata.tsv.gz 1.0 Mb (ftp)(http) TSV
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