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Series GSE151396 Query DataSets for GSE151396
Status Public on Nov 13, 2020
Title A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming [miRNA-seq]
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary MicroRNAs (miRNAs) act in concert with Argonaute (AGO) proteins to broadly repress mRNA targets. After AGO loading, miRNAs generally exhibit slow turnover. An important exception occurs when miRNAs encounter targets with extensive complementarity, which can trigger a process termed target-directed microRNA degradation (TDMD). Prevailing models of TDMD invoke miRNA tailing and trimming as an essential step in the decay mechanism. Here, a genome-wide screen revealed a novel cullin-RING ubiquitin ligase, which we named the DECAY complex, that mediates TDMD. The DECAY complex interacts with AGO proteins, mediates TDMD induced by multiple transcripts, and does not require tailing and trimming to elicit miRNA turnover. Based upon these findings, we propose a model in which the DECAY complex mediates TDMD by promoting proteasomal decay of miRNA-containing complexes.
Overall design Total RNA was isolated from biological triplicates of each cell line using the miRNeasy Mini RNA isolation kit (QIAGEN). Library preparation and next generation sequencing were performed by DNA Link using NEB Next Small RNA Library Prep for Illumina (New England BioLabs).
Contributor(s) Han J, LaVigne CA, Jones BT, Zhang H, Mendell JT
Citation(s) 33184234
Submission date May 28, 2020
Last update date Feb 12, 2021
Contact name Joshua Mendell
Organization name UT Southwestern Medical Center
Department Molecular Biology
Street address 6000 Harry Hines Blvd., NA6.200A
City Dallas
State/province TX
ZIP/Postal code 75390-9148
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (12)
GSM4577195 smallRNAseq_sgNT-1
GSM4577196 smallRNAseq_sgNT-2
GSM4577197 smallRNAseq_sgNT-3
This SubSeries is part of SuperSeries:
GSE151517 A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming
BioProject PRJNA635672
SRA SRP265145

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Supplementary file Size Download File type/resource
GSE151396_NT-CYRANO_low_filtered.csv.gz 22.4 Kb (ftp)(http) CSV
GSE151396_WT-ZSWIM8KO_low_filtered.csv.gz 25.1 Kb (ftp)(http) CSV
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