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Series GSE151475 Query DataSets for GSE151475
Status Public on Jun 18, 2020
Title Circular RNA circGNAQ acts as the sponge of miR-146a-5p to delay endothelial cell senescence and atherosclerosis progression
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Endothelial cell senescence is one of the most important causes of vascular dysfunction and atherosclerosis. Circular RNAs (circRNAs) are endogenous RNA molecules with covalently closed-loop structures, which have been reported to be abnormally expressed in many human diseases. However, the potential role of circRNAs in endothelial cell senescence remains unknown. Here, we compared the expression patterns of circRNAs in young and senescent human endothelial cells using RNA sequencing. Among the differentially expressed endothelial cell senescence-associated circRNAs, we identified circGNAQ, generated by circularization of exons 4 and 5 of the GNAQ gene, as a circRNA showing markedly reduced levels in senescent endothelial cells. CircGNAQ silencing in young endothelial cells triggered senescence, as determined by a rise in senescence-associated β-galactosidase activity, reduced cell proliferation, and suppressed angiogenesis; circGNAQ overexpression showed the opposite effects. Mechanistic studies revealed that circGNAQ acted as an endogenous miR-146a-5p sponge to sequester and inhibit miR-146a-5p activity, leading to increased PLK2 expression. In vivo studies showed that circGNAQ overexpression delayed endothelial cell senescence and atherosclerosis progression in ApoE-/- mice. These results revealed an important role for circGNAQ in cellular senescence and provide novel insight into the role of circRNAs in atherosclerosis progression.
Overall design circRNA sequencing on ribosomal RNA-depleted total RNA from young and senescent endothelial cells (HUVECs and HCAECs)
Contributor(s) Xiong X, Cai M, Wu W, Zhou M
Citation(s) 34552819
Submission date May 29, 2020
Last update date Sep 29, 2021
Contact name weipeng Wu
Organization name Guangdong Medical University
Lab Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Institute of Aging Research
Street address Xin Cheng Avenue 1#, Songshan Lake
City Dongguan
State/province Guangdong
ZIP/Postal code 523808
Country China
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (4)
GSM4579140 Young HUVEC
GSM4579141 Senescent HUVEC
GSM4579142 Young HCAEC
BioProject PRJNA635925
SRA SRP265295

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Supplementary file Size Download File type/resource
GSE151475_All_circRNAs.txt.gz 523.7 Kb (ftp)(http) TXT
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