GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE151742 Query DataSets for GSE151742
Status Public on Jun 04, 2020
Title Expression of the neuronal tRNA n-Tr20 regulates synaptic transmission and seizure susceptibility
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Loss of the nuclear-encoded brain-specific arginine UCU tRNA, n-Tr20, gene increases seizure threshold in mice, and alters inhibitory neurotransmission in the hippocampus. We investigated the molecular impact of loss of n-Tr20 expression on the trancriptome and translatome in the forebrain and hippocampus of multiple n-Tr20 mutant and control strains. Loss of n-Tr20 altered translation initiation by activating the integrated stress response and suppressing mTOR signaling, the latter of which contributes to the enhanced GABAergic transmission. 
Overall design 33 samples are analyzed in this study from n-Tr20 tRNA knockouts and transgenes in the context of C57BL/6J (B6J) and C57BL6/NJ (B6N) forebrain and hippocampus. The design includes 4 replicates each of forebrain RNA-seq from B6N, B6N-nTr20-/-, B6J-nTr20-/-, B6J, and B6J with the B6N n-Tr20 transgene (B6J-Tg(nTr20N/N) or B6J-Tgwt) mice; one sample was sequenced twice to account for batch effects. Additionally, this study includes hippocampus RNA-seq and ribosome profiling with 3 replicates each from B6N and B6N-nTr20-/- hippocampi.
Contributor(s) Kapur M, Ganguly A, Nagy G, Adamson SI, Chuang JH, Frankel WN, Ackerman SL
Citation(s) 32853550
Submission date Jun 03, 2020
Last update date Sep 03, 2020
Contact name Scott Adamson
Organization name The Jackson Laboratory
Lab Chuang
Street address 10 Discovery Drive
City Farmington
State/province CT
ZIP/Postal code 06032
Country USA
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (33)
GSM4589657 B6N_1_batch1_forebrain_RNA
GSM4589658 B6N_2_batch1_forebrain_RNA
GSM4589659 B6N_3_batch1_forebrain_RNA
BioProject PRJNA637076
SRA SRP265841

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151742_RAW.tar 97.7 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap