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Series GSE151900 Query DataSets for GSE151900
Status Public on Oct 11, 2021
Title A Polycomb domain found in committed cells impairs differentiation when introduced into PRC1 in pluripotent cells (EBs/NPCs RNA-seq datasets)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The CBX family of proteins is central to proper mammalian development via key roles in Polycomb-mediated maintenance of repression. CBX proteins in differentiated lineages have chromatin compaction and phase separation activities that might contribute to maintaining repressed chromatin. The predominant CBX protein in pluripotent cells, CBX7, lacks the domain required for these activities. We inserted this functional domain into CBX7 in embryonic stem cells to test the hypothesis that it contributes a key epigenetic function. ESCs expressing this chimeric CBX7 were impaired in their ability to properly form embryoid bodies and neural progenitor cells and showed reduced activation of lineage-specific genes across differentiation. Neural progenitors exhibited a corresponding inappropriate maintenance of Polycomb binding at neural-specific loci over the course of differentiation. We propose that a switch in the ability to compact and phase separate is a central aspect of Polycomb group function during the transition from pluripotency to differentiated lineages.
Overall design Embryonic stem cells expressing different versions of CBX7 (WT, CaPS, mCaPS, KO) were differentiated into embryoid bodies and neural progenitor cells. Gene expression profiles were determined for each cell type across differentiation by performing RNA-Seq on three replicates each.
Contributor(s) Jaensch ES, Kingston RE
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Submission date Jun 05, 2020
Last update date Oct 19, 2021
Contact name Robert Kingston
Organization name Massachusetts General Hospital
Department Molecular Biology
Lab Kingston Lab
Street address 185 Cambridge Street, 7th Floor
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (90)
GSM4593108 RNA_EB_Day0_WT_Rep1
GSM4593109 RNA_EB_Day0_WT_Rep2
GSM4593110 RNA_EB_Day0_WT_Rep3
This SubSeries is part of SuperSeries:
GSE151901 A Polycomb domain found in committed cells impairs differentiation when introduced into PRC1 in pluripotent cells
BioProject PRJNA637609
SRA SRP266147

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151900_CaPS_R_EB_counts.txt.gz 2.2 Mb (ftp)(http) TXT
GSE151900_CaPS_R_NPC_counts.txt.gz 2.2 Mb (ftp)(http) TXT
GSE151900_all_EB_counts.txt.gz 3.5 Mb (ftp)(http) TXT
GSE151900_all_NPC_counts.txt.gz 3.5 Mb (ftp)(http) TXT
GSE151900_hetCaPS_EB_counts.txt.gz 2.5 Mb (ftp)(http) TXT
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Processed data are available on Series record

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