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Series GSE151947 Query DataSets for GSE151947
Status Public on Jul 01, 2021
Title Onecut1 and Otx2 directly regulate multiple genes expressed early in cone and horizontal cell development [scRNA-seq]
Organism Gallus gallus
Experiment type Expression profiling by high throughput sequencing
Summary The development of the retina into a highly organized structure occurs via the production of over 100 cell types from a pool of retinal progenitor cells (RPCs). While some RPCs are capable of making many types of retinal cell types, some terminally dividing RPCs are restricted to the production of specific types of daughter cells. Notably, although such restrictions can be limited to the production of a very specific single cell type, in other cases, production of two very different cell types, such as a photoreceptor and an interneuron, occurs. How specific types of RPCs make specific types of neuron is not fully understood. Combining RNA-seq and ATAC-seq, we compared the transcriptome and chromatin profiles between one type of biased RPC and other types of RPCs. The biased RPC that was studied is one that we previously defined by its expression of a cis-regulatory module (CRM) for the thyroid hormone receptor beta gene (Thrb), the earliest known marker of cone photoreceptors. In this earlier study, we found that Otx2 and Onecut1 drove expression of this Thrb CRM, as well as regulated expression of the Thrb gene. Here we explored the roles of these Otx2 and Oc1 further. We discovered that their roles extend well beyond regulating Thrb. They collaborate to regulate multiple genes important in cone development and/or function, including Rxrg, a known partner of Thrb. We also identified several new enhancers of genes active in developing cones. These data expand our understanding of the gene regulatory network (GRN) for cone development and contribute new CRMs for vectors that are expressed only in cones, as well as enable the genesis of cones from stem cells, for their use in retinal disease cell and gene therapies.
 
Overall design RNA-seq for ThrbPos and ThrbNeg cells in chick
 
Contributor(s) Lonfat N, Wang S, Lee C, Choi J, Park PJ, Cepko C
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Submission date Jun 07, 2020
Last update date Jul 01, 2021
Contact name CHANGHEE LEE
E-mail(s) chlee@genetics.med.harvard.edu
Phone 617-432-6534
Organization name Harvard Medical School
Department Genetics
Lab Clifford Tabin
Street address 77 AVENUE LOUIS PASTEUR
City BOSTON
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (3)
GPL19064 Illumina MiSeq (Gallus gallus)
GPL19787 Illumina NextSeq 500 (Gallus gallus)
GPL26853 Illumina NovaSeq 6000 (Gallus gallus)
Samples (4)
GSM4594235 scRNAseq_ThrbPos_rep1
GSM4594236 scRNAseq_ThrbPos_rep2
GSM4594237 scRNAseq_ThrbNeg_rep1
Relations
BioProject PRJNA637870
SRA SRP266303

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151947_Seurat.rds.gz 65.0 Mb (ftp)(http) RDS
GSE151947_barcodes.tsv.gz 100.7 Kb (ftp)(http) TSV
GSE151947_features.tsv.gz 129.8 Kb (ftp)(http) TSV
GSE151947_matrix.mtx.gz 42.7 Mb (ftp)(http) MTX
GSE151947_meta.tsv.gz 648.5 Kb (ftp)(http) TSV
GSE151947_scRNAseq_gg5_transgene.gtf.gz 10.4 Mb (ftp)(http) GTF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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