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Series GSE152454 Query DataSets for GSE152454
Status Public on May 26, 2021
Title Transcriptome analysis of Arabidopsis thaliana roots, H2O2 and Rose Bengal [20180920_172844_1O_H2O2]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary In order to better understand the context of root water stress and its relationship to other stresses, I undertook an evaluation of osmotic water stress by transcriptome analyses. Whole transcriptome MARSeq (Diego Adhemar Jaitin, 2017) analysis can enable us to study the changes in gene expression (changes in transcript prevalence) in depth under different treatments. It is a cost effective method to develop transcriptional activity. It uses relatively small amounts of RNA and reads sequences from the 3’-polyA containing end. This method can reveal underlying trends that can help us understand the less obvious processes happening in our system. To carry this out I processed whole transcriptome data separately from WT seedling roots exposed to 5 mM H2O2 or 10 µM RB for 0, 1, 2, 3 h or 5 mM H2O2 or 10 µM for 2h .
 
Overall design WT Arabidopsis thaliana seedling roots were exposed to 5 mM H2O2 or 10 µM RB for 0, 1, 2, 3 h or 5 mM H2O2 and 10 µM for 2h ., and root RNA was extracted sequenced using the MARSeq protocol.
 
Contributor(s) Chen T, Fluhr R
Citation(s) 33793947
Submission date Jun 15, 2020
Last update date May 26, 2021
Contact name Tomer Chen
E-mail(s) tomerlivechen@gmail.com
Organization name Weizmann Institute of Science
Department Plant and Environmental Sciences
Street address P.O.box 26
City Rehovot
ZIP/Postal code 7610001
Country Israel
 
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (15)
GSM4616080 RBH R 2a (1O_H2O2)
GSM4616081 RBH R 2b (1O_H2O2)
GSM4616082 RBH R 2c (1O_H2O2)
This SubSeries is part of SuperSeries:
GSE152462 A role for lipoxygenase in the production of 1O2 and promoting the root's response to osmotic stress in Arabidopsi
Relations
BioProject PRJNA639495
SRA SRP267327

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE152454_Deseq_all_results.txt.gz 7.4 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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