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Series GSE152459 Query DataSets for GSE152459
Status Public on May 26, 2021
Title Transcriptome analysis of Arabidopsis thaliana shoots and roots, exposed to PEG-induced osmotic shock, PEG+Histidine, PEG+SHAM [20191204_130529_uLox_PEG_Shock]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary In order to better understand the context of root water stress and its relationship to other stresses, I undertook an evaluation of osmotic water stress by transcriptome analyses. Whole transcriptome MARSeq (Diego Adhemar Jaitin, 2017) analysis can enable us to study the changes in gene expression (changes in transcript prevalence) in depth under different treatments. It is a cost effective method to develop transcriptional activity. It uses relatively small amounts of RNA and reads sequences from the 3’-polyA containing end. This method can reveal underlying trends that can help us understand the less obvious processes happening in our system. To carry this out I processed whole transcriptome data separately from WT and µlox seedling roots and shoots under 30% PEG -induced osmotic shock and control conditions and after 0, 1, 2, 3 h.
Overall design µlox Arabidopsis thaliana seedlings were exposed to 30% PEG, for 0, 1, 2, 3 h, and root RNA was extracted sequenced using the MARSeq protocol.
Contributor(s) Chen T, Fluhr R
Citation(s) 33793947
Submission date Jun 15, 2020
Last update date May 26, 2021
Contact name Tomer Chen
Organization name Weizmann Institute of Science
Department Plant and Environmental Sciences
Street address 26
City Rehovot
ZIP/Postal code 7610001
Country Israel
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (24)
GSM4616194 Ulox_P_0AS (PEG_Shock)
GSM4616195 Ulox_P_0BS (PEG_Shock)
GSM4616196 Ulox_P_0CS (PEG_Shock)
This SubSeries is part of SuperSeries:
GSE152462 A role for lipoxygenase in the production of 1O2 and promoting the root's response to osmotic stress in Arabidopsi
BioProject PRJNA639500
SRA SRP267337

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE152459_Deseq_all_results.txt.gz 16.6 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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