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Series GSE15250 Query DataSets for GSE15250
Status Public on Mar 17, 2009
Title Patterns of micro RNA expression characterize stages of human B-cell differentiation
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Mature B-cell differentiation provides an important mechanism for the acquisition of adaptive immunity. Malignancies derived from mature B-cells constitute the majority of leukemias and lymphomas. These malignancies often maintain the characteristics of the normal B-cells that they are derived from, a feature that is frequently used in their diagnosis. The role of microRNAs in mature B-cells is largely unknown. Through concomitant microRNA and mRNA-profiling, we demonstrate a potential regulatory role for microRNAs at every stage of the mature B-cell differentiation process. Further, we have experimentally identified a direct role for the microRNA-regulation of key transcription factors in B-cell differentiation: LMO2 and PRDM1 (Blimp1). We also profiled microRNA of B-cell tumors derived from diffuse large B cell lymphoma, Burkitt lymphoma and chronic lymphocytic leukemia. We found that in contrast to many other malignancies, common B-cell malignancies do not down-regulate microRNAs, but rather maintain the microRNA-expression patterns of their normal B-cell counterparts. Further, each tumor-type maintained the expression of the lineage-specific microRNAs and expression of these lineage-specific microRNAs could correctly predict the lineage of B-cell malignancies in over 90% of the cases. Thus, our data demonstrate that microRNAs may be important in maintaining the mature B-cell phenotype in normal and malignant B-cells.
 
Overall design Burkitt lymphoma, Chronic Lymphocytic Leukemia (Mutated), Chronic Lymphocytic Leukemia (Unmutated), Activated B cell-like Diffuse large B cell lymphoma, and Germinal Center-like Diffuse large B cell lymphoma Samples,
 
Contributor(s) Jacobs CL, Jima DD, Dave SS
Citation(s) 19202128
Submission date Mar 16, 2009
Last update date Jan 13, 2016
Contact name Dereje D. Jima
E-mail(s) ddjima2014@gmail.com
Phone 9195155932
Organization name North Carolina State University
Department Center for Human Health and the Environment (CHHE) and Bioinformatics Research Center (BRC)
Street address 850 Campus Drive
City Raleigh
State/province NC
ZIP/Postal code 27708
Country USA
 
Platforms (1)
GPL7722 miRCURY LNA microRNA Array, v.10.0 - hsa, mmu & rno
Samples (70)
GSM380895 Burkitt Lymphoma BL 508
GSM380896 Burkitt Lymphoma BL 510
GSM380897 Burkitt Lymphoma BL 513
Relations
BioProject PRJNA116543

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15250_RAW.tar 27.3 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table
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