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Series GSE152535 Query DataSets for GSE152535
Status Public on Jun 28, 2021
Title Requirement of mitochondrial transcription factor A in interleukin-17-producing gdT cells to regulate the small intestine homeostasis and metabolism
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Interleukin 17 (IL-17)-producing gamma delta T (gdT17) cells are one of the major cellular sources of IL-17 under the steady state and in disease. However, gdT17 cell metabolism and its role in tissue homeostasis remain poorly understood. Here, we show that the tissue milieu shapes splenic and intestinal gdT17 cell gene signatures. Conditional deletion of mitochondrial transcription factor A (Tfam) in gdT17 cells significantly affected gdT17 cell maintenance systemically. In vivo deletion of Tfam in gdT17 cells resulted in small intestinal tissue remodeling and increased small intestine length that was caused by the enhanced tuft cell–group 2 innate lymphoid cell (ILC2) circuit in mice. In vitro, IL-22, a cytokine highly produced by gdT17 cells, inhibited IL-13-induced tuft cell differentiation. Mice with Tfam deletion in gdT17 cells also showed dysregulation of the small intestine transcriptome and metabolism with less body weight but enhanced anti-helminth immunity. Thus, our work revealed a critical role of mitochondrial metabolism in gdT17 cell maintenance and in regulating small intestine homeostasis and metabolism.
 
Overall design RNA-seq (3 replicates) analysis of spleeen, small intestine, and large intestine gdT17 cells from Tfam cKO and control mice as well as (3 replicates) analysis of small intestine tissue from Tfam cKO and control mice
 
Contributor(s) Zhou L, Fu Z, Dean J
Citation(s) 34294718
Submission date Jun 15, 2020
Last update date Sep 28, 2021
Contact name Liang Zhou
Organization name University of Florida
Department IDI
Lab V3-207
Street address 2015 SW 16th Ave
City Gainesville
State/province FL
ZIP/Postal code 32608
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (24)
GSM4617731 Sp_Ctr_1
GSM4617732 Sp_Ctr_2
GSM4617733 Sp_Ctr_3
Relations
BioProject PRJNA639599
SRA SRP267414

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE152535_LI_gdT17_STAR_counts.txt.gz 241.4 Kb (ftp)(http) TXT
GSE152535_SI_Tissue_STAR_counts.txt.gz 277.6 Kb (ftp)(http) TXT
GSE152535_SI_gdT17_STAR_counts.txt.gz 252.4 Kb (ftp)(http) TXT
GSE152535_Sp_gdT17_STAR_counts.txt.gz 230.5 Kb (ftp)(http) TXT
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Processed data are available on Series record

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