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| Status |
Public on Mar 18, 2009 |
| Title |
Expression data of induced pluripotent stem cell |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We present a robust serum-free system for the rapid and efficient reprogramming of mouse somatic cells by Oct4, Sox2 and Klf4. The elimination of fetal bovine serum and oncogene c-Myc allowed reprogramming cells to be detected as early as Day 2 and reached greater than 10% of the population at Day 7 post retroviral transduction. The resulting iPS colonies were isolated with high efficiency to establish pluripotent cell lines. Based on this method, we further developed iPS-SF1 as a dedicated reprogramming medium for chemical screening and mechanistic investigations.
Comparasion of iPS cell lines derived from serum-free culture condition, MEF cells cultured in serum and serum-free condition and ES cell lines was shown.
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| |
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| Overall design |
Selected induced pluripotent stem cell lines and its original mouse embroynic fibroblast were comparaed to defined mouse embroynic stem cell lines: R1 and CGR8.
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| Contributor(s) |
Chen J, He W, Zeng L, Pei D |
| Citation(s) |
20595395, 23202127 |
| |
| Submission date |
Mar 17, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Duanqing Pei |
| E-mail(s) |
duanqing.pei@gmail.com
|
| Phone |
086-20-32290520
|
| Fax |
086-20-32290606
|
| Organization name |
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
|
| Department |
Center for Molecular Medicine
|
| Lab |
Lingwen Zeng's Lab
|
| Street address |
Room 301, International Business Incubator, Guangzhou science park
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510663 |
| Country |
China |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA116495 |
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