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| Status |
Public on Mar 20, 2023 |
| Title |
Multiomics profiling of young and old quiescent skeletal muscle stem cells [GSH RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To uncover new pathways that are important for skeletal muscle stem cell (MuSC) aging, we performed multiomics profiling, including transcriptomics, DNA methylomics, proteomics, and metabolomics on quiescent MuSCs from young and old mice. Our goals were to discover pathways that have been overlooked by isolated profiling approaches and to gain insight into which changes are causal, compensatory, correlational, and consequential. In our work, we found that glutathione metabolism is a key pathway of MuSC that involves a compensatory feedback loop. Follow-up experiments showed that old MuSCs actually form a dichotomy between glutathione-high MuSCs and glutathione-low MuSCs. RNA-Seq showed that glutathione-high old MuSCs are able to synthesize adequate glutathione and thus compensate adequately for oxidative stress with increased glutathione turnover, while glutathione-low old MuSCs have failed to compensate for oxidative stress metabolically and instead show increased inflammatory signaling.
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| Overall design |
C57BL/6 male mice were used at two ages: young (4 months) and old (23 months). Quiescent MuSCs were isolated from limb muscles by FACS (CD45- CD31- Sca1- VCAM+). Sorted cells were immediately stained with ThiolTracker Violet (5 uM) to indicate glutathione (GSH) status and resorted by FACS. For young, all cells were recollected (almost all are GSH-high). For old, the population was split into GSH-high and GSH-low collection groups. Each replicate represents the cells from a single animal (young) or pooled two animals (old).
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| Contributor(s) |
Brett JO, Rando TA, Benjamin DI, Both PA |
| Citation(s) |
36854304 |
| Submission date |
Jun 18, 2020 |
| Last update date |
Mar 20, 2023 |
| Contact name |
Jamie O. Brett |
| E-mail(s) |
jamie.o.brett@gmail.com
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| Organization name |
Stanford University
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| Department |
Neurology and Neurological Sciences
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| Lab |
Thomas A. Rando
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| Street address |
3801 Miranda Avenue, Building 100, Room E4-216
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| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE152798 |
Multiomics profiling of young and old quiescent skeletal muscle stem cells |
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| Relations |
| BioProject |
PRJNA640417 |
| SRA |
SRP267894 |
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