|
| Status |
Public on Mar 20, 2023 |
| Title |
Multiomics profiling of young and old quiescent skeletal muscle stem cells [aging WGBS] |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
To uncover new pathways that are important for skeletal muscle stem cell aging, we performed multiomics profiling, including transcriptomics, DNA methylomics, proteomics, and metabolomics on quiescent muscle stem cells from young and old mice. Our goals were to discover pathways that have been overlooked by isolated profiling approaches and to gain insight into which changes are causal, compensatory, correlational, and consequential. In our work, we found that glutathione metabolism is a key pathway of muscle stem cell aging that involves a compensatory feedback loop. Follow-up experiments showed that old muscle stem cells actually form a dichotomy between glutathione-high muscle stem cells and glutathione-low muscle stem cells. RNA-Seq showed that glutathione-high old muscle stem cells are able to synthesize adequate glutathione and thus compensate adequately for oxidative stress with increased glutathione turnover, while glutathione-low old muscle stem cells have failed to compensate for oxidative stress metabolically and instead show increased inflammatory signaling.
|
| |
|
| Overall design |
C57BL/6 male mice were used at two ages: young (4 months) and old (22 months). Quiescent muscle stem cells were isolated from limb muscles by FACS (CD45- CD31- Sca1- VCAM+) and immediately used for analysis. Each replicate represents the muscle stem cells from a single animal. There are 4 replicates in each group.
|
| |
|
| Contributor(s) |
Brett JO, Rando TA |
| Citation(s) |
36854304 |
| Submission date |
Jun 18, 2020 |
| Last update date |
Mar 20, 2023 |
| Contact name |
Jamie O. Brett |
| E-mail(s) |
jamie.o.brett@gmail.com
|
| Organization name |
Stanford University
|
| Department |
Neurology and Neurological Sciences
|
| Lab |
Thomas A. Rando
|
| Street address |
3801 Miranda Avenue, Building 100, Room E4-216
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE152798 |
Multiomics profiling of young and old quiescent skeletal muscle stem cells |
|
| Relations |
| BioProject |
PRJNA640418 |
| SRA |
SRP267895 |
Less...
More...