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| Status |
Public on Sep 30, 2020 |
| Title |
Single cell transcriptomic profile of thymic CD21-CD35- B cells in human neonates |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have demonstrated that thymic B cells, and more concretely CD21-CD35-, are highly heterogeneous in human neonates. To unravel the heterogeneity, we sorted and analyzed the transcriptome by single-cell RNA sequencing the CD21-CD35- B cell subset in six human donors of 4 days and 4 months old.
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| Overall design |
Examination of single cell transcriptome of thymic CD21-CD35- B cells in human neonates by single cell RNA-sequencing.
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| Contributor(s) |
Cordero H, Zorn E |
| Citation(s) |
34599177 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| U01 AI131339 |
Development and significance of the plasma cell niche in the human infant thymus |
Trustees of Columbia University in the City of New York |
Emmanuel Zorn |
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| |
| Submission date |
Jun 23, 2020 |
| Last update date |
Oct 13, 2021 |
| Contact name |
Hector Cordero |
| E-mail(s) |
hc3023@cumc.columbia.edu
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| Organization name |
Columbia University
|
| Street address |
650W 168th Street
|
| City |
New York |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
|
| GSM4634461 |
Single cell thymic CD19+CD21-CD35- [HE001] |
| GSM4634462 |
Single cell thymic CD19+CD21-CD35- [HE002] |
| GSM5471717 |
Single cell thymic CD19+CD21-CD35- [HE005] |
| GSM5471718 |
Single cell thymic CD19+CD21-CD35- [HE006] |
| GSM5471719 |
Single cell thymic CD19+CD21-CD35- [HE007] |
| GSM5471720 |
Single cell thymic CD19+CD21-CD35- [HE008] |
|
| Relations |
| BioProject |
PRJNA641447 |
| SRA |
SRP268530 |
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