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| Status |
Public on Jun 30, 2024 |
| Title |
Splicing regulatory network of master regulator RNA Binding Protein Multiple Splicing (RBPMS) in human Embryonic stem cell derived Vascular Smooth Muscle Cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the splicing and gene expression regulation by RBPMS In hES-VSMCs. We have used a Doxycycline inducible over-expression system to examine the splicing networks controlled by RBPMS in VSMCs; a protein that is highly expressed in native arterial tissue VSMCs. Previously, we have described its splicing regulatory functions in rat VSMCs (Nakagaki-SIlva et al, 2019). Here we report human VSMC specific interactions using VSMCs differentiated from human ES cells via a neural crest lineage. These cells do not endogenously express RBPMS; however, over-expression drives splicing patterns characteristic of mature tissue VSMCs evenin the in vitro differentiated cells.
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| Overall design |
Examination of transcriptomics upon over-expression of a splicing regulator in in vitro differentiated VSMCs.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/39248180/
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| |
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| Contributor(s) |
Jacob AG, Moutsopoulos I, Mohorianu I, Smith C, SInha S |
| Citation(s) |
39248180 |
| |
| Submission date |
Jul 01, 2020 |
| Last update date |
Sep 29, 2024 |
| Contact name |
Irina Mohorianu |
| E-mail(s) |
data-submissions@stemcells.cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Wellcome-MRC Cambridge Stem Cell Institute
|
| Street address |
Puddicombe Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA643486 |
| SRA |
SRP269508 |
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