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Series GSE15388 Query DataSets for GSE15388
Status Public on Mar 25, 2009
Title EZH1 and H3K27me3 ChIP-chip data from mouse embryonic stem cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary Trimethylation on H3K27 (H3K27me3) mediated by Polycomb repressive complex 2 (PRC2) has been linked to embryonic stem cell (ESC) identity and pluripotency. EZH2, the catalytic subunit of PRC2, has been reported as the sole histone methyltransferase that methylates H3K27 and mediates transcriptional silencing. Analysis of Ezh2(-/-) ESCs suggests existence of an additional enzyme(s) catalyzing H3K27 methylation. We have identified EZH1, a homolog of EZH2 that is physically present in a noncanonical PRC2 complex, as an H3K27 methyltransferase in vivo and in vitro. EZH1 colocalizes with the H3K27me3 mark on chromatin and preferentially preserves this mark on development-related genes in Ezh2(-/-) ESCs. Depletion of Ezh1 in cells lacking Ezh2 abolishes residual methylation on H3K27 and derepresses H3K27me3 target genes, demonstrating a role of EZH1 in safeguarding ESC identity. Ezh1 partially complements Ezh2 in executing pluripotency during ESC differentiation, suggesting that cell-fate transitions require epigenetic specificity.
 
Overall design We used ChIP-chip to reveal genome-wide locationation of Polycomb protein EZH1 and H3K27me3 mark in undifferentiated wild-type (CJ7) and Ezh2-/- mutant embryonic stem (ES) cells. DNA fragments pulled down by chromatin immunoprecipitation, amplified and hybridization on Affymetrix mouse Tiling 2.0R array sets.
 
Contributor(s) Shen X, Orkin S
Citation(s) 19026780
Submission date Mar 25, 2009
Last update date Mar 21, 2017
Contact name Xiaohua Shen
E-mail(s) shen@bloodgroup.tch.harvard.edu
Phone 617-632-6496
Organization name Dana Farber Cancer Institute
Department Pediatric Oncology
Lab Stuart Orkin lab
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (7)
GPL6444 [Mm35b_P01R] Affymetrix Mouse Tiling 2.0R Set, Array 1
GPL6445 [Mm35b_P02R] Affymetrix Mouse Tiling 2.0R Set, Array 2
GPL6446 [Mm35b_P03R] Affymetrix Mouse Tiling 2.0R Set, Array 3
Samples (28)
GSM386188 H3K27me3 ChIP in CJ7 cells on A chip
GSM386189 H3K27me3 ChIP in CJ7 cells on B chip
GSM386190 H3K27me3 ChIP in CJ7 cells on C chip
Relations
BioProject PRJNA117125

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15388_RAW.tar 3.9 Gb (http)(custom) TAR (of CEL, TXT)
Processed data provided as supplementary file
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