GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE154270 Query DataSets for GSE154270
Status Public on Dec 31, 2020
Title Methylation and gene expression data obtained from Dnmt3b+/- peripheral T-cell lymphomas.
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Dnmt3b is a tumor suppressor in oncogene-driven lymphoid and myeloid malignancies in mice. However, it is poorly understood whether reduced Dnmt3b activities can initiate malignant hematopoiesis. We modulated Dnmt3b activity in vivo by generating Dnmt3b+/− mice expressing one wild-type allele. Here, we analyzed methylation and gene expression in Dnmt3b+/- peripheral T-cell lymphomas (PTCLs).
Overall design We performed WGBS and RNA-seq analysis on Dnmt3b+/- peripheral T-cell lymphoma samples isolated from lymph nodes of terminally sick mice. WGBS experiments were performed in biological duplicates, RNA-seq experiments in triplicates. In addition we performed WGBS of Dnmt3a -/- PTCL.
Contributor(s) Lopusna K, Nowialis P, Opavska J, Abraham A, Riva A, Haney SL, Opavsky R
Citation(s) 33450231
Submission date Jul 12, 2020
Last update date Mar 31, 2021
Contact name Rene Opavsky
Phone 3522948599
Organization name University of FLORIDA
Department Anatomy and cell biology
Street address 2033 Mowry rd, CGRC r..265
City Gainesville
State/province FL
ZIP/Postal code 32610
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM4668122 WGBS Dnmt3b+/- 1
GSM4668123 WGBS Dnmt3b+/- 2
GSM4668124 RNAseq Dnmt3b+/- 1
BioProject PRJNA645699
SRA SRP271469

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE154270_RAW.tar 267.2 Mb (http)(custom) TAR (of BIGWIG)
GSE154270_RNAseq_FPKM.txt.gz 1.8 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap