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Series GSE15434 Query DataSets for GSE15434
Status Public on Jan 01, 2010
Title Gene expression profiling in AML with normal karyotype: A multicenter study investigating molecular markers in 251 cases
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Acute myeloid leukemia (AML) is a heterogeneous disease and AML with normal karyotype (AML-NK) is categorized as an intermediate-risk group. Over the past years molecular analyses successfully identified biomarkers that will further allow to dissecting clinically meaningful subgroups in this disease. Thus far, somatic mutations were identified which elucidate the disturbance of cellular growth, proliferation, and differentiation processes in hematopoietic progenitor cells. In AML-NK, acquired gene mutations with prognostic relevance were identified for FLT3, CEBPA, and NPM1. FLT3-ITD mutations were associated with short relapse-free and overall survival, while mutations in CEBPA or NPM1 (without concomitant FLT3-ITD) had a more favorable outcome.
Here, we present a multicenter study investigating gene expression profiles of 251 cases of AML-NK. In three centers whole-genome microarray analyses were performed to delineate robust and common expression signatures for molecular markers in AML-NK.
Overall design All samples were obtained from untreated patients at the time of diagnosis. Cells used for microarray analysis were collected from the purified fraction of mononuclear cells after Ficoll density centrifugation (Dresden, n=78; Munich, n=97; Ulm, n=77). Each laboratory performed its routine diagnostic algorithms, including the characterization of molecular markers.
Contributor(s) Kohlmann A, Bullinger L, Thiede C, Schaich M, Schnittger S, Döhner K, Dugas M, Klein H, Döhner H, Ehninger G, Haferlach T
Citation(s) 20003504
Submission date Mar 27, 2009
Last update date Mar 25, 2020
Contact name Hans-Ulrich Klein
Organization name Columbia University Medical Center
Department Neurology
Lab Center for Translational and Computational Neuroimmunology
Street address 622 W 168th St
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (251)
GSM387060 AML with normal karyotype #1
GSM387061 AML with normal karyotype #2
GSM387062 AML with normal karyotype #3
BioProject PRJNA115791

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Supplementary file Size Download File type/resource
GSE15434_RAW.tar 1.3 Gb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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