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Series GSE154473 Query DataSets for GSE154473
Status Public on May 26, 2021
Title DEAD-box RNA helicase 27-Mediated MicroRNA Biogenesis Is Essential for Zygote Division and Stem Cell Homeostasis in Arabidopsis
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Zygote division and stem cell homeostasis are crucial events for plant development. Here, we found that mutations in DEAD-box RNA helicase 27 (RH27) affected zygote division and stem cell homeostasis in Arabidopsis (Arabidopsis thaliana). The strong mutant allele rh27-1 showed a zygote-lethal phenotype, while the weak mutant allele rh27-2 showed defective embryogenesis and compromised stem cell homeostasis in both the shoot apical meristem (SAM) and root apical meristem (RAM). RH27 was expressed in embryos from the zygote stage, and in the SAM and RAM. Cytological analyses revealed that stem cell homeostasis in the SAM and RAM was disrupted in rh27-2. Then, the expression levels of genes related to stem cell homeostasis were elevated in rh27-2, which coincides with down-regulations of their corresponding negative regulatory microRNAs. Furthermore, small RNA-sequencing indicated that the reduction of miRNAs was a global effect in both shoot apices and root tips of rh27-2. Additionally, the accumulation of pri-miRNAs was detected to be decreased. Biochemical studies showed that the nucleus-localized RH27 interacted directly with SERRATE, a component of the microprocessor complex, and DAWDLE, which facilitates the recruitment of substrates to the complex, suggesting that RH27 is a subunit of the microprocessor complex. Overall, this work provides evidences that RH27 regulates zygote division and stem cell homeostasis in Arabidopsis through miRNA biogenesis.
Overall design Small RNA-sequencing and RNA-sequencing were used to study global effects of RH27 on miRNA accumulation and expressions of pri-miRNAs and miRNA targets,respectively.
Contributor(s) Hou X, Sun J, Song X, Cao X, Liu C
Citation(s) 33751089
Submission date Jul 15, 2020
Last update date May 28, 2021
Contact name Jing Sun
Organization name Chinese Academy of Sciences
Department IGDB
Street address No. 1 West Beichen Road
City Beijing
ZIP/Postal code 100101
Country China
Platforms (2)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
GPL26208 Illumina NovaSeq 6000 (Arabidopsis thaliana)
Samples (24)
GSM4671825 WT_shoot apices_sRNA-seq_rep1
GSM4671826 WT_shoot apices_sRNA-seq_rep2
GSM4671827 WT_shoot apices_sRNA-seq_rep3
BioProject PRJNA646453
SRA SRP272058

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GSE154473_RAW.tar 1.5 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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