GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE155105 Query DataSets for GSE155105
Status Public on Sep 03, 2020
Title Chemical genetics approach identifies ABNORMAL INFLORESCENCE MERISTEM 1 as a putative target of a novel sulfonamide that protects Arabidopsis against photorespiratory stress [RNA-seq]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Alterations of hydrogen peroxide (H2O2) levels have a profound impact on numerous signaling cascades orchestrating stress responses, plant growth and development, including programmed cell death. To expand the repertoire of known molecular mechanisms implicated in H2O2 signaling, we performed a forward chemical screen to identify small molecules that could alleviate the photorespiratory-induced cell death phenotype of Arabidopsis thaliana mutants lacking H2O2 scavenging capacity by peroxisomal CATALASE2. Here, we report the characterization of pakerine, a m-sulfamoyl benzamide from the sulfonamide family. Pakerine alleviates the cell death phenotype of cat2 mutants exposed to photorespiration-promoting conditions and delays dark-induced senescence in wild type Arabidopsis leaves. By using a combination of transcriptomics, metabolomics and affinity purification we identified ABNORMAL INFLORESCENCE MERISTEM 1 (AIM1) as a putative protein target of pakerine. AIM1 is a 3-hydroxyacyl-CoA dehydrogenase involved in β-fatty acid oxidation that contributes to jasmonic acid (JA) and salicylic acid (SA) biosynthesis. Whereas intact JA biosynthesis was not required for pakerine bioactivity, our results point towards a role for β-oxidation-dependent SA production in execution of H2O2-mediated cell death.
Overall design Gene expression analysis of CATALASES2 deficient Arabidopsis thaliana plants exposed to a combinatorial treatment of photorespiratory inducing growth conditions and a chemical treatment of a chemical alleviating the enhanced cell death phenotype of the mutant plants. Four different treatments are applied to seven day old plants (3 biological replicates per treatment) grown in liquid 1/2 MS media in translucent 96-well plates. Treatments include 1. mock (DMSO) treated plants grown in long day conditions (16 h/8 h light/dark, 100 µmol m-2 s-1 light intensity, 21 oC, 70% relative humidity), 2. mock treated plants grown for 2 days in photorespiratory stress conditions (24 hour light 100 µmol m-2 s-1 light intensity, 21 oC, 70% relative humidity), 3. Chemical treatment, consisting of Pakerine dissolved in DMSO (10 μM final conc.) grown in long day conditions, 4. Chemical treatment, consisting of Pakerine dissolved in DMSO (10 μM final conc.) grown in photorespiratory stress conditions.
Contributor(s) van der Meer T, Kerchev PI, van Breusegem F, Stevens CV, Verlee A, Willems P, Impens F, Gevaert K, Testerink C
Citation(s) 32887516
Submission date Jul 24, 2020
Last update date Nov 02, 2020
Contact name Tom van der Meer
Organization name Wageningen University
Street address Droevendaalsesteeg 1
City Wageningen
ZIP/Postal code 6708PB
Country Netherlands
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (12)
GSM4695496 cat2_ctrl_DMSO_1
GSM4695497 cat2_ctrl_DMSO_2
GSM4695498 cat2_ctrl_DMSO_3
BioProject PRJNA648424
SRA SRP273472

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE155105_RNAseq_processed_Data.xlsx 7.3 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap