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Status |
Public on Sep 03, 2020 |
Title |
Chemical genetics approach identifies ABNORMAL INFLORESCENCE MERISTEM 1 as a putative target of a novel sulfonamide that protects Arabidopsis against photorespiratory stress [RNA-seq] |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Alterations of hydrogen peroxide (H2O2) levels have a profound impact on numerous signaling cascades orchestrating stress responses, plant growth and development, including programmed cell death. To expand the repertoire of known molecular mechanisms implicated in H2O2 signaling, we performed a forward chemical screen to identify small molecules that could alleviate the photorespiratory-induced cell death phenotype of Arabidopsis thaliana mutants lacking H2O2 scavenging capacity by peroxisomal CATALASE2. Here, we report the characterization of pakerine, a m-sulfamoyl benzamide from the sulfonamide family. Pakerine alleviates the cell death phenotype of cat2 mutants exposed to photorespiration-promoting conditions and delays dark-induced senescence in wild type Arabidopsis leaves. By using a combination of transcriptomics, metabolomics and affinity purification we identified ABNORMAL INFLORESCENCE MERISTEM 1 (AIM1) as a putative protein target of pakerine. AIM1 is a 3-hydroxyacyl-CoA dehydrogenase involved in β-fatty acid oxidation that contributes to jasmonic acid (JA) and salicylic acid (SA) biosynthesis. Whereas intact JA biosynthesis was not required for pakerine bioactivity, our results point towards a role for β-oxidation-dependent SA production in execution of H2O2-mediated cell death.
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Overall design |
Gene expression analysis of CATALASES2 deficient Arabidopsis thaliana plants exposed to a combinatorial treatment of photorespiratory inducing growth conditions and a chemical treatment of a chemical alleviating the enhanced cell death phenotype of the mutant plants. Four different treatments are applied to seven day old plants (3 biological replicates per treatment) grown in liquid 1/2 MS media in translucent 96-well plates. Treatments include 1. mock (DMSO) treated plants grown in long day conditions (16 h/8 h light/dark, 100 µmol m-2 s-1 light intensity, 21 oC, 70% relative humidity), 2. mock treated plants grown for 2 days in photorespiratory stress conditions (24 hour light 100 µmol m-2 s-1 light intensity, 21 oC, 70% relative humidity), 3. Chemical treatment, consisting of Pakerine dissolved in DMSO (10 μM final conc.) grown in long day conditions, 4. Chemical treatment, consisting of Pakerine dissolved in DMSO (10 μM final conc.) grown in photorespiratory stress conditions.
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Contributor(s) |
van der Meer T, Kerchev PI, van Breusegem F, Stevens CV, Verlee A, Willems P, Impens F, Gevaert K, Testerink C |
Citation(s) |
32887516 |
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Submission date |
Jul 24, 2020 |
Last update date |
Nov 02, 2020 |
Contact name |
Tom van der Meer |
E-mail(s) |
tom.vandermeer@wur.nl
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Organization name |
Wageningen University
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Street address |
Droevendaalsesteeg 1
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City |
Wageningen |
ZIP/Postal code |
6708PB |
Country |
Netherlands |
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Platforms (1) |
GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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Samples (12)
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Relations |
BioProject |
PRJNA648424 |
SRA |
SRP273472 |
Supplementary file |
Size |
Download |
File type/resource |
GSE155105_RNAseq_processed_Data.xlsx |
7.3 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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