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| Status |
Public on Dec 20, 2020 |
| Title |
Effect of short-term hyperglycemia on primary fetoplacental endothelial cells (fpEC) isolated from human placenta arteries |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In order to investigate the effect of in vitro hyperglycemia on gene expression of fpEC, cells were treated with hypergylcemia (25mM) for 24h.
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| Overall design |
5 different isolations of primary fpEC (800,000) were split in two gelatin coated 75cm2 culture flasks in EBM medium. After 24h, medium was replaced either by normoglycemic EBM (5.5mM D-glucose) or with EBM containing 25mM D-glucose. After 24h, RNA was isolated using TRI REAGENT. 5µg RNA of all control conditions and 5µg of each hyperglycemic treatment was pooled and the pools used for the microarray experiment.
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| Contributor(s) |
Hiden U, Cvitic S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 28, 2020 |
| Last update date |
Dec 21, 2020 |
| Contact name |
Ursula Hiden |
| Organization name |
Medical University of Graz
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| Department |
Obstetrics and Gynecology
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| Street address |
Auenbruggerplatz 14
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| City |
Graz |
| ZIP/Postal code |
8036 |
| Country |
Austria |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA649213 |
