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Series GSE155356 Query DataSets for GSE155356
Status Public on Jul 30, 2020
Title Charaterization of monocytes/macrophages function in Club cell regeneration after naphthalene injury in WT mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary During repair phase after naphathalene injury 3 subsets of myeloid cells accumulate at the site of injury including P1 (monoyte), P2 (monocyte-derived macrophages and P3 (resident alternatively activated airway macrophages). To better understand the function of each subset in epithelial repair, myeloid cell subsets were flow-sorted from lungs of WT mice treated with naphtahelen for 6 days and RNA-seq analysis was performed
Overall design C57Bl/6J female mice (9-10 weeks) were treatment with naphthalene at a dose of 200-250 mg/kg. On day 6, mice were euthanized and their lungs were collected and digested for myeloid cell sorting of P1 (CD11b+ ly6c+ cells), P2 (CD11b+ CD11c+ F4/80+ cells) and P3 (Siglrc-F+ CD206+ CD11c+ F4/80+ cells). then P1-P3 were RNAseq analysis.
Contributor(s) Dagher R, Wang J
Citation(s) 32963227
Submission date Jul 29, 2020
Last update date Oct 13, 2020
Contact name Jingya Wang
Phone 3013980977
Organization name Astrazeneca
Department Early respiratory and immunology
Street address 1 Medimmune Way
City Gaithersburg
State/province Maryland
ZIP/Postal code 20878
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (11)
GSM4700001 Day0_sham_naïve_1
GSM4700002 Day0_sham_naïve_2
GSM4700003 Day0_sham_naïve_3
This SubSeries is part of SuperSeries:
GSE155361 IL-33-ST2 axis regulates myeloid cell differentiation and activation facilitating effective club cell regeneration
BioProject PRJNA649517
SRA SRP274113

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE155356_RAW.tar 1.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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