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Series GSE155356 Query DataSets for GSE155356
Status Public on Jul 30, 2020
Title Charaterization of monocytes/macrophages function in Club cell regeneration after naphthalene injury in WT mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary During repair phase after naphathalene injury 3 subsets of myeloid cells accumulate at the site of injury including P1 (monoyte), P2 (monocyte-derived macrophages and P3 (resident alternatively activated airway macrophages). To better understand the function of each subset in epithelial repair, myeloid cell subsets were flow-sorted from lungs of WT mice treated with naphtahelen for 6 days and RNA-seq analysis was performed
 
Overall design C57Bl/6J female mice (9-10 weeks) were treatment with naphthalene at a dose of 200-250 mg/kg. On day 6, mice were euthanized and their lungs were collected and digested for myeloid cell sorting of P1 (CD11b+ ly6c+ cells), P2 (CD11b+ CD11c+ F4/80+ cells) and P3 (Siglrc-F+ CD206+ CD11c+ F4/80+ cells). then P1-P3 were RNAseq analysis.
 
Contributor(s) Dagher R, Wang J
Citation(s) 32963227
Submission date Jul 29, 2020
Last update date Oct 13, 2020
Contact name Jingya Wang
E-mail(s) jingya.wang@astrazeneca.com
Phone 3013980977
Organization name Astrazeneca
Department Early respiratory and immunology
Street address 1 Medimmune Way
City Gaithersburg
State/province Maryland
ZIP/Postal code 20878
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (11)
GSM4700001 Day0_sham_naïve_1
GSM4700002 Day0_sham_naïve_2
GSM4700003 Day0_sham_naïve_3
This SubSeries is part of SuperSeries:
GSE155361 IL-33-ST2 axis regulates myeloid cell differentiation and activation facilitating effective club cell regeneration
Relations
BioProject PRJNA649517
SRA SRP274113

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE155356_RAW.tar 1.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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