GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE155359 Query DataSets for GSE155359
Status Public on Jul 30, 2020
Title Charaterization of function of ST2 receptor on macrophages function during Club cell regeneration after naphthalene injury in WT mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary During repair phase after naphathalene injury 3 subsets of myeloid cells accumulate at the site of injury including P1 (monoyte), P2 (monocyte-derived macrophages and P3 (resident alternatively activated airway macrophages). ST2 receptor was found to be expressed by P2 and P3 subsets. To understand which biological processes in lung macrophages were regulated by ST2, myeloid cell subsets were flow-sorted from Naphthalene-treated WT and ST2-/- mice on d6 and their transcriptome analyzed using RNA-seq
Overall design ST2Ko and C57Bl/6J female mice (9-10 weeks) were treatment with naphthalene at a dose of 200-250 mg/kg. On day 6, mice were euthanized and their lungs were collected and digested for myeloid cell sorting of P1 (CD11b+ ly6c+ cells), P2 (CD11b+ CD11c+ F4/80+ cells) and P3 (Siglrc-F+ CD206+ CD11c+ F4/80+ cells). then P1-P3 were RNAseq analysis.
Contributor(s) Dagher R, Wang J
Citation(s) 32963227
Submission date Jul 29, 2020
Last update date Oct 13, 2020
Contact name Jingya Wang
Phone 3013980977
Organization name Astrazeneca
Department Early respiratory and immunology
Street address 1 Medimmune Way
City Gaithersburg
State/province Maryland
ZIP/Postal code 20878
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (18)
GSM4700031 WT_1_P1
GSM4700032 WT_1_P2
GSM4700033 WT_1_P3
This SubSeries is part of SuperSeries:
GSE155361 IL-33-ST2 axis regulates myeloid cell differentiation and activation facilitating effective club cell regeneration
BioProject PRJNA649519
SRA SRP274117

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE155359_RAW.tar 1.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap