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Series GSE155871 Query DataSets for GSE155871
Status Public on Apr 14, 2021
Title Temporarily epigenetic repression in Bergmann glia regulates the migration of Granule cells (RNA-seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Forming tight interaction with both Purkinje and granule cells (GCs), Bergmann glia (BG) are essential for cerebellar morphogenesis and neuronal homeostasis. However, how BG act in this process is unclear without comprehensively transcriptomic landscape of BG. Here, high temporal-resolution investigation of transcriptomes with FACS-sorted BG revealed the dynamic of genes within given functions and pathways enabled BG to assist neural migration and construct neuron-glia network. We found the peak time of GCs migration (P7-10) was strikingly coincided with the downregulation of extracellular matrix (ECM) related genes by prevailing transcriptional repression, and the disrupting of which by Setdb1 ablation at P7-10 in BG led to the significant migration defect of GCs by abnormal ECM expression emphasizing the criticality of Nfix-Setdb1 mediated H3K9me3 repressive complex for the precise regulation of GCs migration in vivo. Thus, BG’s transcriptomic landscapes offer an insight into the mechanism by which BG are in-depth integrated in cerebellar neural network.
 
Overall design BGs mRNA profiles of Nestin-∆TK-IRES-GFP or Mash1 cre-ERT2, R26-LSL-tdTomato mice were generated by deep sequencing using BGI-500
 
Contributor(s) Zhang B
Citation(s) 34026436
Submission date Aug 07, 2020
Last update date Jul 14, 2021
Contact name shaoxuan chen
E-mail(s) sxchen@stu.xmu.edu.cn
Phone 18250876513
Organization name xiamen university
Street address School of Life Sciences, Xiang'an Campus, Xiamen University, Xiang'an District
City Xiamen
State/province Fujian Province
ZIP/Postal code 361102
Country China
 
Platforms (1)
GPL23479 BGISEQ-500 (Mus musculus)
Samples (27)
GSM4714916 P1BGwt1_1
GSM4714917 P1BGwt2_1
GSM4714918 P4BGwt1_1
This SubSeries is part of SuperSeries:
GSE155873 Temporarily epigenetic repression in Bergmann glia regulates the migration of Granule cells
Relations
BioProject PRJNA655904
SRA SRP276660

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE155871_all_sample_expression.txt.gz 4.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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