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Status |
Public on Apr 14, 2021 |
Title |
Temporarily epigenetic repression in Bergmann glia regulates the migration of Granule cells (RNA-seq) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Forming tight interaction with both Purkinje and granule cells (GCs), Bergmann glia (BG) are essential for cerebellar morphogenesis and neuronal homeostasis. However, how BG act in this process is unclear without comprehensively transcriptomic landscape of BG. Here, high temporal-resolution investigation of transcriptomes with FACS-sorted BG revealed the dynamic of genes within given functions and pathways enabled BG to assist neural migration and construct neuron-glia network. We found the peak time of GCs migration (P7-10) was strikingly coincided with the downregulation of extracellular matrix (ECM) related genes by prevailing transcriptional repression, and the disrupting of which by Setdb1 ablation at P7-10 in BG led to the significant migration defect of GCs by abnormal ECM expression emphasizing the criticality of Nfix-Setdb1 mediated H3K9me3 repressive complex for the precise regulation of GCs migration in vivo. Thus, BG’s transcriptomic landscapes offer an insight into the mechanism by which BG are in-depth integrated in cerebellar neural network.
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Overall design |
BGs mRNA profiles of Nestin-∆TK-IRES-GFP or Mash1 cre-ERT2, R26-LSL-tdTomato mice were generated by deep sequencing using BGI-500
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Contributor(s) |
Zhang B |
Citation(s) |
34026436 |
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Submission date |
Aug 07, 2020 |
Last update date |
Jul 14, 2021 |
Contact name |
shaoxuan chen |
E-mail(s) |
sxchen@stu.xmu.edu.cn
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Phone |
18250876513
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Organization name |
xiamen university
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Street address |
School of Life Sciences, Xiang'an Campus, Xiamen University, Xiang'an District
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City |
Xiamen |
State/province |
Fujian Province |
ZIP/Postal code |
361102 |
Country |
China |
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Platforms (1) |
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Samples (27)
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This SubSeries is part of SuperSeries: |
GSE155873 |
Temporarily epigenetic repression in Bergmann glia regulates the migration of Granule cells |
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Relations |
BioProject |
PRJNA655904 |
SRA |
SRP276660 |