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Series GSE156236 Query DataSets for GSE156236
Status Public on Oct 14, 2022
Title Transcriptional profiling of striatum and cortex from a LacO-Q140 inducible mouse model of Huntington’s disease with early and late mutant HTT lowering
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Inducible mutant huntingtin mice were genetically engineered by introducing the lac operon sequence (LacO) into the Q140 promoter of the Q140 KI mice. LacO-140Q mice were then crossed to transgenic Lac repressor protein (LacIR) mice. LacIR occupies the LacO sequence in the promoter and thereby blocks transcription of Q140. Beta-galactoside analog isopropyl thiogalactoside (IPTG) binds to LacIR, which results in decreased affinity between the repressor and operator, thereby relieving repression. All mice in this study have the LacIR transgene, therefore the default state of the mouse containing LacOQ140;LacIR is maximal repression of Q140. CHDI generated and analyzed RNASeq data from inducible LacOQ140 mice at 2 points: 6 and 12 months. Transcriptional profiling in the striatum and cortex with early versus late mutant HTT lowering is detailed.
 
Overall design Hdh(LacO-140Q/+):β-actin-LacIR tg or Hdh(LacO-+/+):β-actin-LacIR tg wild-type mice received either no treatment or ОІ-D-1-Thiogalactopyranoside (IPTG) treatment starting at E5 and continued to 2 months or 8 months of age or throughout life. RNASeq analysis was performed on samples from the striatum and cortex at 6 and 12 months of age. N = 10 per group (5F/5M). There are 4 groups at 6 months of age and 5 groups at 12 months of age.
 
Contributor(s) Aaronson J, Rosinski J
Citation(s) 36278490
Submission date Aug 13, 2020
Last update date Oct 25, 2022
Contact name Jeff Aaronson
E-mail(s) jeff.aaronson@chdifoundation.org
Phone (609) 945-9616
Organization name CHDI
Department Discovery & Systems Biology
Street address 155 Village Boulevard, Suite 200
City Princeton
State/province New Jersey
ZIP/Postal code 08540
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (217)
GSM4727234 LacQ140I (*) 12 months IPTG to 2 months Cortex female mRNA rep1
GSM4727235 LacQ140I (*) 12 months IPTG to 2 months Cortex female mRNA rep2
GSM4727236 LacQ140I (*) 12 months IPTG to 2 months Cortex female mRNA rep3
Relations
BioProject PRJNA657116
SRA SRP277466

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE156236_laco12m_counts_unswapped.txt.gz 6.8 Mb (ftp)(http) TXT
GSE156236_laco6m_counts.txt.gz 6.2 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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