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Series GSE156294 Query DataSets for GSE156294
Status Public on Mar 27, 2024
Title in situ Hi-C profiles of DP thymocyltes from control and Suv39h1/Suv39h2 double knockout mice
Organism Mus musculus
Experiment type Other
Summary H3K9me3-dependent heterochromatin is critical for the silencing of repeat-rich pericentromeric regions and also has key roles in repressing lineage-inappropriate protein-coding genes for healthy cellular function. Here we investigate the disruption of genome organization in the absence of H3K9me3-dependent heterochromatin by performing in situ Hi-C in primary immune cells deficient in both Suv39h1 and Suv39h2 (Suv39DKO), the major mammalian histone methyltransferase enzymes which catalyse heterochromatic H3K9me3 deposition.
Overall design CD4+CD8+ double positive mouse thymocyte cells were extracted from two Suv39h1+/y Suv39h2+/- (control) mice and two Suv39h1-/y Suv39h2-/- double knock-out (Suv39DKO) mice and profiled by in situ Hi-C. All samples correspond to individual mice.
Contributor(s) Keenan CR, Coughlan HD, Smyth GK, Allan RS
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Submission date Aug 14, 2020
Last update date Mar 28, 2024
Contact name Gordon K Smyth
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM4728474 Control, replicate 1
GSM4728475 Control, replicate 2
GSM4728476 Suv39h1, Suv39h2 double knock-out, replicate 1
This SubSeries is part of SuperSeries:
GSE156297 RNA-seq, LaminB1 ChIP-seq and in situ Hi-C of double positive thymocytes from control and Suv39h1/Suv39h2 double knockout mice
BioProject PRJNA657285
SRA SRP277564

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Supplementary file Size Download File type/resource
GSE156294_GenomicRegions.bed.gz 497.0 Kb (ftp)(http) BED
GSE156294_RAW.tar 677.2 Mb (http)(custom) TAR (of MTX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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