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Series GSE157061 Query DataSets for GSE157061
Status Public on Mar 10, 2021
Title Genome-wide analysis of differentially expressed miRNAs and their associated regulatory networks in lenses deficient for the congenital cataract-linked tudor domain containing protein TDRD7
Platform organism synthetic construct
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Mutations/deficiency of TDRD7, which encodes a tudor domain containing protein involved in post-transcriptional gene expression control, causes early-onset cataract in humans and animal models. While Tdrd7 is implicated in the control of key lens mRNAs, the impact of Tdrd7 deficiency on microRNAs (miRNAs), and how this contributes to cataracts, is undefined. Here, we address this critical knowledge-gap by investigating Tdrd7-targeted knockout (Tdrd7-/-) mice that exhibit fully penetrant juvenile cataracts. To gain global insights into miRNA profile alterations, we performed Affymetrix miRNA 3.0 microarray analysis on Tdrd7-/- mouse lenses at postnatal day (P) 4, because this stage precedes cataract formation and thus informs on early miRNA misexpression while minimizing detection of secondary changes.
Overall design miRNA microarray comparision of lenses fromTdrd7 targeted germline knockout (KO) mouse line (Tdrd7tm1.1Chum, or Tdrd7-/-), and wild-type control
Contributor(s) Anand D, Lachke SA
Citation(s) 33665188
Submission date Aug 28, 2020
Last update date Mar 10, 2021
Contact name Deepti Anand
Organization name University of Delaware
Department Biological Sciences
Street address 351 Wolf Hall, University of Delaware
City Newark
State/province Delaware
ZIP/Postal code 19716
Country USA
Platforms (1)
GPL16384 [miRNA-3] Affymetrix Multispecies miRNA-3 Array
Samples (6)
GSM4751837 KO1
GSM4751838 KO2
GSM4751839 KO3
BioProject PRJNA660001

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Supplementary file Size Download File type/resource
GSE157061_RAW.tar 25.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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