 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 30, 2009 |
| Title |
Gene expression changes in Bmi1 knock-out MEFs as compared to wild-type. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Polycomb group (PcG) proteins control organism development by regulating the expression of developmental genes. Transcriptional regulation by PcG proteins is achieved at least partly through the PRC2-mediated methylation on lysine 27 of histone H3 (H3K27) and PRC1-mediated ubiquitylation on lysine 119 of histone H2A (uH2A). As an integral component of PRC1, Bmi1 has been demonstrated to be critical for H2A ubiquitylation. Although recent studies have revealed the genome wide binding patterns of some of the PRC1 and PRC2 components, as well as the H3K27me3 mark, there have been no reports describing genome wide localization of uH2A. Using the recently developed ChIP-Seq technology, here we report genome wide localization of the Bmi1-dependent uH2A mark in MEF cells. Gene promoter averaging analysis indicates a peak of uH2A just inside the transcription start site (TSS) of well annotated genes. This peak is enriched at promoters containing the H3K27me3 mark and represents the least expressed genes in WT MEF cells. In addition, peak finding reveals regions of local uH2A enrichment throughout the mouse genome, including almost 700 gene promoters. Genes with promoter peaks of uH2A exhibit lower level expression when compared to genes that do not contain promoter peaks of uH2A. Moreover, we demonstrate that genes with uH2A peaks have increased expression upon Bmi1 knockout. Importantly, local enrichment of uH2A is not limited to regions containing the H3K27me3 mark. We provide evidence to suggest that DNA methylation is tightly linked to H2A ubiquitylation in high density CpG promoters. Thus, our work not only reveals Bmi1-dependent H2A ubiquitylation but also suggests that uH2A targeting in differentiated cells may employ a different mechanism from that in ES cells.
|
| |
|
| Overall design |
RNA was extracted from wild-type MEF cells which were immortalized through TBX2 overexpression, amplified, labeled, and hybridized to an Affymetrix 430 2 microarray. In parallel, RNA was prepared from TBX2-immortalized Bmi1 null MEF cells and hybridized to an Affymetrix 430 2 microarray.
|
| |
|
| Contributor(s) |
Kallin EM, Cao R, Jothi R, Xia K, Cui K, Zhao K, Zhang Y |
| Citation(s) |
19503595 |
| |
| Submission date |
Apr 16, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Eric M. Kallin |
| E-mail(s) |
eric.kallin@crg.es
|
| Organization name |
Center for Genomic Regulation
|
| Department |
Differentiation and Cancer
|
| Lab |
Thomas Graf
|
| Street address |
C/ Dr. Aiguader, 88
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE15909 |
Gene expression and UH2A ChIP-Seq binding analysis in Bmi1 knock-out and wild type MEFs |
|
| Relations |
| BioProject |
PRJNA122989 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15715_RAW.tar |
8.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
