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Series GSE15725 Query DataSets for GSE15725
Status Public on Jun 02, 2009
Title Chromosomal duplication in the grandifolia-D mutants of Arabidopsis thaliana, tiling array data
Organism Arabidopsis thaliana
Experiment type Genome variation profiling by genome tiling array
Summary The number of cells in an organ is a major factor for the determination of organ size. However, genetic basis of cell number determination is not well understood. Three grandifolia-D (gra-D) mutants of Arabidopsis thaliana developed huge leaves containing two- to three-fold increased number of cells of the wild type. Tiling array and microarray analysis of gra-D mutants suggested that genes found in a lower part of chromosome 4 were upregulated, suggesting the occurrence of segmental chromosomal duplications in the gra-D mutants. These region contain positive regulators of cell proliferation such as AINTEGUMENTA (ANT) and cyclin genes such as CYCD3;1.
 
Overall design genomic DNA was extracted using DNeasy plant mini kit (Qiagen) from vegetative part of seedlings grown in vitro. DNA from wild type (wt, control) and the 3 grandifolia mutants (gra1-D, gra2-D and gra3-D) were submitted to tiling array hybridization.
 
Contributor(s) Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H
Citation(s) 19508432
Submission date Apr 17, 2009
Last update date May 09, 2017
Contact name Nathalie Gonzalez
E-mail(s) nagon@psb.vib-ugent.be
Phone +32 (0)9 331 39 56
Organization name VIB
Department Plant System Biology
Lab System Biology of Yield
Street address Technologiepark 927
City Gent
ZIP/Postal code 9052
Country Belgium
 
Platforms (1)
GPL10977 [At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array
Samples (1)
GSM393555 gra2-D
This SubSeries is part of SuperSeries:
GSE15726 Chromosomal duplication in the grandifolia-D mutants of Arabidopsis thaliana
Relations
BioProject PRJNA122999

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15725_RAW.tar 71.7 Mb (http)(custom) TAR (of BAR, BED, CEL)
Processed data provided as supplementary file

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