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Series GSE15770 Query DataSets for GSE15770
Status Public on May 20, 2009
Title WT and Get1 +/- Bladder Time Course
Organism Mus musculus
Experiment type Expression profiling by array
Summary Skin and bladder epithelia form effective permeability barriers through the activation of distinct differentiation gene programs. Employing a genome-wide gene expression study, we identified transcription regulators whose expression correlates highly with that of differentiation markers both in bladder and skin, including the Grainyhead factor Get1/Grhl3, already known to be important for epidermal barrier formation. In the bladder, Get1 is most highly expressed in the differentiated umbrella cells and its mutation in mice leads to a defective bladder epithelial barrier formation due to failure of apical membrane specialization. Genes encoding components of the specialized urothelial membrane, the uroplakins, were downregulated in Get1-/- mice. At least one of these genes, Uroplakin II, is a direct target of Get1. The urothelial-specific activation of the Uroplakin II gene is due to selective binding of Get1 to the Uroplakin II promoter in urothelial cells, most likely regulated by histone modifications. These results demonstrate a key role for Get1 in urothelial differentiation and barrier formation.
 
Overall design To gain insights into common and unique transcriptional regulatory programs during bladder differentiation, we profiled global gene expression in whole mouse bladder at E14.5, E16.5, and E18.5.
 
Contributor(s) Yu Z, Mannik J, Soto A, Lin K, Andersen B
Citation(s) 19494835
Submission date Apr 21, 2009
Last update date Feb 11, 2019
Contact name Amelia Soto Hopkin
E-mail(s) sotoa@uci.edu
Phone 9498249372
Organization name University of California Irvine
Department Biological Chemistry & Medicine
Lab Bogi Andersen
Street address 250 Sprague Hall
City Irvine
State/province CA
ZIP/Postal code 92617
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM394789 E18.5 Get1 +/- Bladder 2-4
GSM395744 E18.5 Get1 +/- Bladder 2-5
GSM395745 E18.5 Get1 +/- Bladder 2-6
This SubSeries is part of SuperSeries:
GSE16150 Get1 in urothelial differentiation and barrier formation
Relations
BioProject PRJNA123041

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15770_RAW.tar 27.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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