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Status |
Public on Nov 13, 2020 |
Title |
The lipogenic regulator SREBF2 induces Transferrin in circulating melanoma cells and suppresses ferroptosis [single cell RNA-Seq] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Circulating tumor cells (CTCs) are shed by cancer into the bloodstream, where a viable subset overcomes oxidative stress to initiate metastatic outgrowth. Here we show that CTCs from patients with BRAF-mutant melanoma coordinately upregulate both lipogenesis and iron homeostasis pathways. In clonally-derived cultures of melanoma CTCs these pathways are correlated with both intrinsic and acquired resistance to BRAF inhibitors. The lipogenesis regulator SREBF2 directly induces transcription of the iron carrier Transferrin (TF), reducing intracellular iron pools, reactive oxygen species (ROS) and lipid peroxidation, and conferring resistance to both BRAF inhibitors and inducers of ferroptosis. Knockdown of endogenous TF impairs tumor formation by melanoma CTCs, and their tumorigenic defects are partially rescued by the lipophilic anti-oxidants Ferrostatin-1 and Vitamin E. In a cohort of patient-derived melanoma CTCs, single cell RNA-seq identifies a subset with high lipogenic, iron metabolic and proliferative signatures, which are correlated with adverse clinical outcome, irrespective of treatment regimen. Thus, SREBF2-driven iron homeostatic pathways contribute to cancer progression, drug resistance and metastasis.
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Overall design |
We undertook single cell RNA-Seq of individually picked melanoma CTCs following microfluidic enrichment from primary blood specimens (PMID: 23552373, PMID: 24577360). We picked 76 individual CTCs freshly isolated from 22 melanoma patients and validated them to be melanoma cells by their expression of melanoma lineage markers (PMID: 29453278). We also picked and performed single cell RNA-Seq on 20 single CTCs that had been incubated in culture medium for 4-8 weeks, a condition in which only viable CTCs persist, but before they initiate in vitro proliferation. We also performed single cell RNA-Seq on six individually selected leukocytes from a healthy donor.
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Contributor(s) |
Hong X, Roh W, Sullivan RJ, Wong KH, Wittner BS, Guo H, Dubash TD, Sade-Feldman M, Wesley BK, Horwitz E, Boland GM, Marvin DL, Bonesteel T, Lu C, Aguet F, Burr R, Freeman SS, Parida L, Calhoun K, Jewett MK, Nieman LT, Hacohen N, Naar AM, Ting DT, Toner M, Stott SL, Getz G, Maheswaran S, Haber DA |
Citation(s) |
33203734 |
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Submission date |
Sep 09, 2020 |
Last update date |
Nov 23, 2020 |
Contact name |
Ben S. Wittner |
E-mail(s) |
wittner.ben@mgh.harvard.edu
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Organization name |
Massachusetts General Hospital
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Department |
Center for Cancer Research
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Lab |
Lawrence
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Street address |
149 13th Street
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02129 |
Country |
USA |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (102)
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This SubSeries is part of SuperSeries: |
GSE157745 |
The lipogenic regulator SREBF2 induces Transferrin in circulating melanoma cells and suppresses ferroptosis |
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Relations |
BioProject |
PRJNA662604 |
SRA |
SRP281893 |
Supplementary file |
Size |
Download |
File type/resource |
GSE157743_TPM.single_cell_RNA_Seq.csv.gz |
2.2 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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