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Series GSE15825 Query DataSets for GSE15825
Status Public on Jan 04, 2010
Title An mRNA/3' UTR-directed primary piRNA pathway in Drosophila ovarian somatic cells
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary Piwi-interacting RNAs (piRNAs) are ~24-30 nucleotide regulatory RNAs that are abundantly expressed in gonads. The most well-understood piRNAs mediate post-transcriptional defense against transposable elements (TEs), and derive from sense or antisense strands as a consequence of "ping-pong" amplification of complementary sequences of active TEs and piRNA master control transcripts. Another class of piRNAs, such as those expressed in pachytene testis, derive from large intergenic clusters that are strictly single-stranded. Here, we report a third substrate that generates abundant primary piRNAs. In somatic follicle cells of Drosophila ovaries, we cloned >1 million piRNAs from thousands of messenger RNAs, and these were quite preferentially derived from 3' untranslated regions. This segregation implies a competition between the translation machinery and primary piRNA biogenesis machinery for mRNA access.
Overall design 3 replicates.
Contributor(s) Balla S, Lau N, Lai EC
Citation(s) 20022248
Submission date Apr 25, 2009
Last update date Aug 28, 2018
Contact name Nicolas Robine
Organization name Sloan-Kettering Institute
Department Developmental Biology
Lab Dr. Eric Lai's Lab
Street address 430, East 67th Street, RRL 517
City New York
State/province NY
ZIP/Postal code 10065
Country USA
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (3)
GSM397756 Drosophila OSS cells_1
GSM397757 Drosophila OSS cells_2
GSM397758 Drosophila OSS cells_3
BioProject PRJNA116747

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15825_RAW.tar 4.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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