GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE158310 Query DataSets for GSE158310
Status Public on Sep 01, 2021
Title De novo DNA methylation suppresses aberrant fate trajectory during epiblast transition [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Genome remethylation is essential for mammalian development. Here we examined cell fate in the absence of de novo DNA methyltransferases. We found that embryonic stem (ES) cells deficient for Dnmt3a and Dnmt3b are rapidly eliminated from chimaeras. Pluripotency progression is derailed towards extra-embryonic trophoblast. This aberrant trajectory is propelled by failure to methylate and suppress expression of Ascl2 during formative transition. Ascl2 deletion rescues transition and improves contribution to chimaeric epiblast but mutant cells are progressively lost in later development. These findings indicate that methylation constrains transcriptome trajectories during developmental transitions by silencing potentially disruptive genes.

This submission is RNA-seq.
Overall design RNA were collected from undifferentiated ES cells and formative EpiLCs from Dnmt3a/3b double knockout cells with control
Contributor(s) Kinoshita M, Li MA, Barber M, Dietmann S, Smith A
Citation(s) 34518230
Submission date Sep 21, 2020
Last update date Nov 03, 2021
Contact name Irina Mohorianu
Organization name University of Cambridge
Department Wellcome-MRC Cambridge Stem Cell Institute
Street address Puddicombe Way
City Cambridge
ZIP/Postal code CB2 0AW
Country United Kingdom
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (4)
GSM4797285 WT.AFKD2
GSM4797286 KO.AFKD2
GSM4797287 WT.2iL
This SubSeries is part of SuperSeries:
GSE158347 Disabling de novo DNA methylation in embryonic stem cells allows an illegitimate fate trajectory
BioProject PRJNA664787
SRA SRP284289

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE158310_cells.tsv.gz 65.1 Kb (ftp)(http) TSV
GSE158310_expression.tsv.gz 39.1 Mb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap