NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE15911 Query DataSets for GSE15911
Status Public on Sep 15, 2009
Title Actinobacillus pleuropneumoniae during a natural infection in pigs
Platform organism Actinobacillus pleuropneumoniae serovar 5b str. L20
Sample organism Actinobacillus pleuropneumoniae
Experiment type Expression profiling by array
Genome variation profiling by array
Summary Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, a respiratory disease which causes great economic losses worldwide. Many virulence factors are involved in the pathogenesis, namely capsular polysaccharides, RTX toxins, LPS and many iron acquisition systems. In order to identify genes that are expressed in vivo during a natural infection, we undertook transcript profiling experiments with an A. pleuropneumoniae DNA microarray, after recovery of bacterial mRNAs from serotype 5b-infected porcine lungs.
 
Overall design Comparative Genomic Hybridizations between Actinobacillus pleuropneumoniae serotype 5b strain L20 (ref) and serotype 5b fresh field isolate 896-07, recovered from infected pig lung tissues following natural acute infection. Two condition transcript profiling experiments : infectious 5b field strain isolated directly from lungs of naturally deceased pigs after acute infection vs infectious 5b field strain grown in BHI broth to an OD600 of 0.300.
 
Contributor(s) Deslandes V, Denicourt M, Harel J, Nash JH, Jacques M
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Apr 30, 2009
Last update date Mar 21, 2012
Contact name Vincent Deslandes
E-mail(s) vincent.deslandes@umontreal.ca
Organization name Université de Montréal - Faculté de Médecine Vétérinaire
Department Groupe de Recherche sur les Maladies Infectieuses du Porc
Street address 3200 rue Sicotte
City St-Hyacinthe
State/province Quebec
ZIP/Postal code J2S7C6
Country Canada
 
Platforms (1)
GPL6658 AppChip2 (NRC-IBS Actinobacillus pleuropneumoniae L20)
Samples (5)
GSM399435 896-07 vs L20 1 (CGH)
GSM399436 896-07 vs L20 2 (CGH)
GSM399461 896-07 in lung tissues vs 896-07 BHI broth 1 (expression)
Relations
BioProject PRJNA116907

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15911_RAW.tar 1.4 Mb (http)(custom) TAR (of MEV)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap