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Status |
Public on Sep 15, 2009 |
Title |
Actinobacillus pleuropneumoniae during a natural infection in pigs |
Platform organism |
Actinobacillus pleuropneumoniae serovar 5b str. L20 |
Sample organism |
Actinobacillus pleuropneumoniae |
Experiment type |
Expression profiling by array Genome variation profiling by array
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Summary |
Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, a respiratory disease which causes great economic losses worldwide. Many virulence factors are involved in the pathogenesis, namely capsular polysaccharides, RTX toxins, LPS and many iron acquisition systems. In order to identify genes that are expressed in vivo during a natural infection, we undertook transcript profiling experiments with an A. pleuropneumoniae DNA microarray, after recovery of bacterial mRNAs from serotype 5b-infected porcine lungs.
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Overall design |
Comparative Genomic Hybridizations between Actinobacillus pleuropneumoniae serotype 5b strain L20 (ref) and serotype 5b fresh field isolate 896-07, recovered from infected pig lung tissues following natural acute infection. Two condition transcript profiling experiments : infectious 5b field strain isolated directly from lungs of naturally deceased pigs after acute infection vs infectious 5b field strain grown in BHI broth to an OD600 of 0.300.
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Contributor(s) |
Deslandes V, Denicourt M, Harel J, Nash JH, Jacques M |
Citation missing |
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Submission date |
Apr 30, 2009 |
Last update date |
Mar 21, 2012 |
Contact name |
Vincent Deslandes |
E-mail(s) |
vincent.deslandes@umontreal.ca
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Organization name |
Université de Montréal - Faculté de Médecine Vétérinaire
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Department |
Groupe de Recherche sur les Maladies Infectieuses du Porc
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Street address |
3200 rue Sicotte
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City |
St-Hyacinthe |
State/province |
Quebec |
ZIP/Postal code |
J2S7C6 |
Country |
Canada |
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Platforms (1) |
GPL6658 |
AppChip2 (NRC-IBS Actinobacillus pleuropneumoniae L20) |
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Samples (5)
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GSM399462 |
896-07 in lung tissues vs 896-07 BHI broth 2 (expression) |
GSM399463 |
896-07 in lung tissues vs 896-07 BHI broth 3 (expression) |
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Relations |
BioProject |
PRJNA116907 |