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Series GSE15925 Query DataSets for GSE15925
Status Public on Jul 01, 2009
Title Mature thymic NKT and T cells
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Invariant Natural killer T (iNKT) cells are a separate lineage of T lymphocytes with innate effector functions. They express an invariant TCR specific for lipids presented by CD1d and their development and effector differentiation rely on a unique gene expression program. We asked whether this program includes microRNAs, small non-coding RNAs that regulate gene expression posttranscriptionally and play key role in the control of cellular differentiation programs. We identified a miRNA profile specific for iNKT cells, which exhibits features of activated/effector T lymphocytes.
 
Overall design In this experiment we compared microRNAs of NKT cells versus those of conventional T lymphocytes, both extracted from wild type mouse thymus.
To produce the populations we enriched mature thymocytes and then sorted NKT cells and conventional T cells (from one sorting we obtain one “NKT” sample and one “T” sample). We performed the experiment in triplicate; the sample NKT 1 was sorted the same day of the sample T 1, NKT 2 with T 2, NKT 3 with T 3.
We used a common reference approach for the 6 samples; as a common reference we produced RNA from total thymocytes, without the enrichment for mature cells and without the sorting; we pooled thymocytes derived from all the thymi used in the study.
The aim of the experiment was to demonstrate that NKT cells have a microRNA profile different from that of conventional T cells.
 
Contributor(s) Dellabona P, Casorati G
Citation(s) 19625646
Submission date May 01, 2009
Last update date May 10, 2016
Contact name Maya Fedeli
E-mail(s) fedeli.maya@hsr.it
Phone 00390226434735
Organization name San Raffaele Scientific Institute
Department DIBIT
Lab Experimental Immunology Unit
Street address via Olgettina 58
City Milan
State/province MI
ZIP/Postal code 20132
Country Italy
 
Platforms (1)
GPL7723 miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno
Samples (6)
GSM399626 NKT 1
GSM399628 NKT 2
GSM399629 NKT 3
Relations
BioProject PRJNA116935

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15925_RAW.tar 9.2 Mb (http)(custom) TAR (of TXT)
GSE15925_legends_v2.pdf.gz 91.2 Kb (ftp)(http) PDF
Processed data included within Sample table

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