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Series GSE15925 Query DataSets for GSE15925
Status Public on Jul 01, 2009
Title Mature thymic NKT and T cells
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Invariant Natural killer T (iNKT) cells are a separate lineage of T lymphocytes with innate effector functions. They express an invariant TCR specific for lipids presented by CD1d and their development and effector differentiation rely on a unique gene expression program. We asked whether this program includes microRNAs, small non-coding RNAs that regulate gene expression posttranscriptionally and play key role in the control of cellular differentiation programs. We identified a miRNA profile specific for iNKT cells, which exhibits features of activated/effector T lymphocytes.
Overall design In this experiment we compared microRNAs of NKT cells versus those of conventional T lymphocytes, both extracted from wild type mouse thymus.
To produce the populations we enriched mature thymocytes and then sorted NKT cells and conventional T cells (from one sorting we obtain one “NKT” sample and one “T” sample). We performed the experiment in triplicate; the sample NKT 1 was sorted the same day of the sample T 1, NKT 2 with T 2, NKT 3 with T 3.
We used a common reference approach for the 6 samples; as a common reference we produced RNA from total thymocytes, without the enrichment for mature cells and without the sorting; we pooled thymocytes derived from all the thymi used in the study.
The aim of the experiment was to demonstrate that NKT cells have a microRNA profile different from that of conventional T cells.
Contributor(s) Dellabona P, Casorati G
Citation(s) 19625646
Submission date May 01, 2009
Last update date May 10, 2016
Contact name Maya Fedeli
Phone 00390226434735
Organization name San Raffaele Scientific Institute
Department DIBIT
Lab Experimental Immunology Unit
Street address via Olgettina 58
City Milan
State/province MI
ZIP/Postal code 20132
Country Italy
Platforms (1)
GPL7723 miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno
Samples (6)
GSM399626 NKT 1
GSM399628 NKT 2
GSM399629 NKT 3
BioProject PRJNA116935

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15925_RAW.tar 9.2 Mb (http)(custom) TAR (of TXT)
GSE15925_legends_v2.pdf.gz 91.2 Kb (ftp)(http) PDF
Processed data included within Sample table

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